Abstract
Oxidative stress is an imbalance between radical-generating and radical-scavenging activity, resulting in oxidation products and tissue damage. This study was aimed to evaluate the status of oxidative stress indices in blood of camels naturally infested with S. scabiei. Forty-seven male camels (Camelus dromedaries) were divided according to the extent of the infested area with Sarcoptes scabiei into four groups, mild (MID, n = 12), moderate (MOD, n = 10), severely infested (SEV, n = 10) and healthy control group (n = 15). Blood was used for determination of red cell count (RBC), hemoglobin (Hb), packed cell volume (PCV), serum nitric oxide (NO•, a free radical), ascorbate and albumin concentrations, and erythrocytic values of malondialdehyde (MDA, a marker of lipid peroxidation), protein carbonyls (PC, an indicator of protein oxidation), glutathione (GSH) superoxide dismutase (SOD) and catalase (CAT). Decreased levels (P < 0.05) of RBC, Hb, PCV, albumin and ascorbate were noticed in MOD and SEV compared to controls with the lowest values (P < 0.05) in SEV except for ascorbate, where MOD did not differ from SEV. Compared to controls, NO• gradually increased (P < 0.05) in MID followed by MOD and SEV, whereas MDA and PC were higher (P < 0.05) in MOD and SEV. PC was higher (P < 0.05) in MOD than SEV. In addition, the antioxidants GSH, SOD and CAT were higher (P < 0.05) in MID and lower (P < 0.05) in MOD and SEV compared to controls. GSH was lower (P < 0.05) in SEV compared to MOD. Besides, Hb was negatively correlated with NO• (r = −0.68, P < 0.001), MDA (r = −0.53, P < 0.001) and PC (r = −0.73, P < 0.001). In conclusion, dromedary sarcoptosis is accompanied by a state of oxidative stress process, which increased by increasing the area of infestation, and may contribute to the pathogenesis of the disease.
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Allen BW, Demchenko IT, Piantadosi CA (2009) Two faces of nitric oxide: implications for cellular mechanisms of oxygen toxicity. J Appl Physiol 106:662–667
Amer AA, Abou El-Ela A, Ratib HZ (2006) Some Hematobiochemical studies on sarcoptic mange infested camels before and after treatment by doramectin at Assiut governorate. Proceedings of the international scientific conference on camels, 9–11 May 2006, KSA. pp 686–691
Arlian LG, Vyszenski-Moher DL, Rapp CM, Hull BE (1996) Production of IL-1 alpha and IL-1 beta by human skin equivalents parasitized by Sarcoptes scabiei. J Parasitol 82:719–723
Arlian LG, Morgan MS, Neal JS (2004) Extracts of scabies mites (Sarcoptidae: Sarcoptes scabiei) modulate cytokine expression by human peripheral blood mononuclear cells and dendritic cells. J Med Entomol 41:69–73
Beers RF Jr, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888
Bickers DR, Athar M (2006) Oxidative stress in the pathogenesis of skin disease. J Invest Dermatol 126:2565–2575
Brown A (2004) Camel—a review of diseases in central Australia. Northern Territory Department of Primary Industry, Fisheries and Mines, Darwin, Technical Bulletin 314:1–16
Brunet LR (2001) Nitric oxide in parasitic infections. Int Immunopharmacol 1:1457–1467
Camkerten I, Sahin T, Borazan G, Gokcen A, Erel O, Das A (2009) Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Vet Parasitol 161:106–109
Çimen MYB (2008) Free radical metabolism in human erythrocytes. Clin Chim Acta 390:1–11
Ciolino HP, Levine RL (1997) Modification of proteins in endothelial cell death during oxidative stress. Free Radic Biol Med 22:1277–1282
Dalle-Donne I, Aldini G, Carini M, Colombo R, Rossi R, Milzani A (2006) Protein carbonylation, cellular dysfunction, and disease progression. J Cell Mol Med 10:389–406
Deloach JR, Wright FC (1981) Ingestion of rabbit erythrocytes containing 51Cr-labeled hemoglobin by psoroptes species, that originated in cattle, mountain sheep or rabbits. J Med Entomol 18:345–348
Dimri U, Sharma MC (2004) Effects of sarcoptic mange and its control with oil of Cedrus deodara, Pongamia glabra, Jatropha curcas and Benzyl Benzoate, both with and without ascorbic acid on growing sheep: epidemiology; assessment of clinical, haematological, cell-mediated humoral immune responses and pathology. J Vet Med A 51:71–78
Dimri U, Sharma MC, Swarup D, Ranjan R, Kataria M (2008) Alterations in hepatic lipid peroxides and antioxidant profile in Indian water buffaloes suffering from sarcoptic mange. Res Vet Sci 85:101–105
Dimri U, Sharma MC, Yamdagni A, Ranjan R, Zama MMS (2010) Psoroptic mange infestation increases oxidative stress and decreases antioxidant status in sheep. Vet Parasitol 68:318–322
Ding AH, Nathan CF, Stuehr DJ (1988) Release of reactive nitrogen intermediates and reactive oxygen intermediates from peritoneal macrophages. J Immunol 141:2407–2412
Feri B, England L, Ames BN (1989) Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natal Acad Sci USA 86:6377–6381
Gorakh M, Sena DS, Rajender K, Mal G, Kumar R (2001) Serum biochemical observations on mange in camels. Ind Vet J 78:104–106
Gutteridge JC (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41:1819–1828
Halliwell B, Chirico S (1993) Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr 57(suppl):715–725S
Herold S, Rock G (2003) Reactions of deoxy-oxy-, and methaemoglobin with nitrogen monoxide. J Biol Chem 278:6623–6634
Hicks MI, Elston DM (2009) Scabies. Dermatologic Therapy 22:279–292
Jain NC (1986) Hematologic techniques. In: Jain NC (ed) Schalm’s veterinary hematology, 3rd edn. Lea and Febiger, Philadelphia, pp 20–86
Kumar D, Raisinghani PM, Manohar GS (1992) Sarcoptic mange in camel: a review. Proceeding of the first international camel conference, Dubai, UAE, 2–6 February 1992. pp. 79–82
Lalli PN, Morgan MS, Arlian LG (2004) Skewed Th1/Th2 immune response to sarcoptes scabiei. J Parasitol 90:711–714
Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478
Loban A, Kime R, Powers H (1997) Iron-binding antioxidant potential of plasma albumin. Clin Sci (Lond) 93:445–451
McClain D, Dana AN, Goldenberg G (2009) Mite infestations. Dermatologic Therapy 22:327–346
McCormick DB, Green HL (1994) Vitamins. In: Burtis CA, Ashwood ER (eds) Tietz textbook of clinical chemistry, 2nd edn. Saunders, Philadelphia, pp 1275–1316
McMillan DC, Powell CL, Bowman ZS, Morrow JD, Jollow DJ (2005) Lipids versus proteins as major targets of pro-oxidant, direct-acting hemolytic agents. Toxicol Sci 88:274–283
Misra HP, Fridovich I (1972) The role of superoxide anion in the auto oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3174
Mohamed HE, Beynen AC (2002) Ascorbic acid content of blood plasma, erythrocytes, leukocytes and liver in camels (Camelus dromedarius) without or with parasite infections. Int J Vitam Nutr Res 72:369–371
Niki E (2009) Lipid peroxidation: physiological levels and dual biological effects. Free Radic Biol Med 47:469–484
Niwa Y, Sumi H, Kawahira K, Terashima T, Nakamura T, Akamatsu H (2003) Protein oxidative damage in the stratum corneum: evidence for a link between environmental oxidants and the changing prevalence and nature of atopic dermatitis in Japan. Br J Dermatol 149:248–54
Pegram RJ, Higgins AJ (1992) Camel ectoparasites: a review. Proceedings of the first international camel conference, Dubai, UAE, 2–6 February 1992. pp 69–78
Placer ZA, Cushman LL, Johnson BC (1966) Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem 16:359–364
Portugal M, Barak V, Ginsburg I, Kohen R (2007) Interplay among oxidants, antioxidants, and cytokines in skin disorders: present status and future considerations. Biomed Pharmacother 61:412–422
Radi R, Peluff G, Alvarez MN, Naviliat M, Cayota A (2001) Unraveling peroxynitrite formation in biological systems. Free Rad Biol Med 30:463–488
Roche M, Rondeau P, Singh NR, Tarnus E, Bourdon E (2008) The antioxidant properties of serum albumin. FEBS Lett 582:1783–1787
Salar-Amoli J, Hejazy M, Ali Esfahani T (2009) Comparison between some oxidative Stress Biomarkers values in serum and plasma of clinically healthy adult camels (Camelus dromedarius) in Iran. Vet Res Commun 33:849–854
Serarslan G, Yılmaz HR, Söğüt S (2005) Serum antioxidant activities, malondialdehyde and nitric oxide levels in human cutaneous leishmaniasis. Clin Exp Dermatol 30:267–271
Skerratt LF, Middleton D, Beveridge I (1999) Distribution of life cycle stages of Sarcoptes scabiei var wombati and effects of severe mange on common wombats in Victoria. J Wildl Dis 35:633–46
Soulsby EJL (1982) Helminths, arthropods and protozoa of domestic animals, 7th edn. Bailliere Tindall, London
Suchitra Sena D, Mal G, Kumar R, Singhvi NM, Chiroania BL, Sahani MS (1999) Clinico-haematological and theraputic studies on mange in camels. Ind Vet J 76:998–1000
Tategaki A, Okuda T, Kawamoto S, Ono K, Shigeta S (2003) Induction of inducible nitric oxide synthase mRNA expression and nitric oxide production from macrophages stimulated with high-molecular size mite antigen HM1. Allergol Int 52:97–103
Trouba KJ, Hamadeh HK, Amin RP, Germolec DR (2002) Oxidative stress and its role in skin disease. Antioxid Redox Signal 4:665–673
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84
Walton SF, Beroukas D, Roberts-Thomson P, Currie BJ (2008) New insights into disease pathogenesis in crusted (Norwegian) scabies: the skin immune response in crusted scabies. Br J Dermatol 158:1247–1255
Yeum KJ, Russell RM, Krinsky NI, Aldini G (2004) Biomarkers of antioxidant capacity in the hydrophilic and lipophilic compartments of human plasma. Arch Biochem Biophys 430:97–103
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Saleh, M.A., Mahran, O.M. & Bassam Al-Salahy, M. Circulating oxidative stress status in dromedary camels infested with sarcoptic mange. Vet Res Commun 35, 35–45 (2011). https://doi.org/10.1007/s11259-010-9450-x
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DOI: https://doi.org/10.1007/s11259-010-9450-x