Skip to main content
SpringerLink
Log in

High-fat feeding and staphylococcus intermedius infection impair beta cell function and insulin sensitivity in mongrel dogs

  • Original Article
  • Published:
Veterinary Research Communications Aims and scope Submit manuscript

Abstract

As obesity is a state of low-grade inflammation, we aimed to investigate the combined effect of high-fat diet and bacterial infection on β-cell function and insulin sensitivity in dogs. We used 20 healthy, male, mongrel dogs randomly divided into four groups: control group—healthy, non-obese dogs; infected group—non-obese dogs with experimentally induced infection (Staphylococcus intermedius); obese group—obese dogs (after 90 day high-fat diet) and obese-infected group—obese dogs with experimentally induced infection (Staphylococcus intermedius). To evaluate insulin sensitivity and β-cell function an intravenous glucose tolerance test (IVGTT) was performed. Plasma insulin increased in all group after glucose infusion. The lowest values were found in obese-infected group. Blood glucose also increased on 3 min after glucose infusion and then gradually decreased. In obese-infected group glucose concentration on 30 min was still significantly higher than initial levels, while in other groups glucose concentration returned to the initial values. The lowest rate of glucose elimination was found in infected group. In dogs of obese group and obese-infected group AUCins 0–60 min was lower compared to controls. AUCglucose 0–60 min values were lowest in infected group, while in obese-infectd group values were the highest. Levels of ∆I/∆G in dogs of obese-infected group were significantly lower compared to controls and infected group. In conclusion, these results reveal that infection in obese dogs leads to impaired glucose tolerance, which is result of impairment in both insulin secretion and insulin sensitivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ahren, Bo, Pacini, G., 2002. Insufficient islet compensation to insulin resistance vs. reduced glucose effectiveness in glucose-intolerant mice. American Journal of Physiology—Endocrinology and Metabolism, 283, E738–E744.

    CAS  PubMed  Google Scholar 

  • Bergman, R. N., 1989. Toward physiological understanding of glucose tolerance. Diabetes, 38, 1512–1527.

    Article  CAS  PubMed  Google Scholar 

  • Bergman, R. N., Ader, M., Huecking K., Citters, G. V., 2002. Accurate assessment of β-cell function the hyperbolic correction. Diabetes, 51, suppl. 1., S212–S220.

    Article  CAS  PubMed  Google Scholar 

  • Cavaghan, M. K., Ehrmann, D. A., Polonsky, K. S., 2000. Interactions between insulin resistance and insulin secretion in the development of glucose intolerance. Journal of Clinical Investigation, 106, 329–333.

    Article  CAS  PubMed  Google Scholar 

  • Chisholm, K. W. and O’Dea, K., 1987. Effect of short-term consumption of high fat diet on glucose tolerance and insulin sensitivity in rats. Journal of Nutritional Science and Vitaminology, 33, 377–390.

    CAS  PubMed  Google Scholar 

  • Festa, A., Williams, K., Hanley, A.J, Haffner, S.M, 2008. Beta-cell dysfunction in subjects with impaired glucose tolerance and early type 2 diabetes: comparison of surrogate markers with first-phase insulin secretion from an intravenous glucose tolerance test. Diabetes, 57, 1638–1644.

    Article  CAS  PubMed  Google Scholar 

  • Frank, G., Anderson, W., Pazak, H., Hodgkins, E., Ballam, J. and Laflamme, D. P., 2001. Use of a high-protein diet in the management of feline diabetes mellitus. Veterinary Therapeutics, 2, 238–246.

    CAS  PubMed  Google Scholar 

  • German, A. J., 2000. The growing problem of obesity in dog and cats. Journal of Small Animal Practice, 41, 530–534.

    Article  Google Scholar 

  • Hays, N., Galassetti, P. and Coker, R., 2008. Prevention and treatment of type 2 diabetes: Current role of lifestyle, natural product, and pharmacological intervention. Pharmacology and Therapeutics, 118, 181–191.

    Article  CAS  PubMed  Google Scholar 

  • Hoenig, M., 2002. Comparative aspects of diabetes mellitus in dogs and cats. Molecular and Cellular Endocrinology, 197, 221–229.

    Article  CAS  PubMed  Google Scholar 

  • Hull, R. L., Kodama, K., Utzschneider, K. M., Carr, D. B., Prigeon, R. L., Kahn, S. E., 2005. Dietary-fat-induced obesity in mice results in beta cell hyperplasia but not increased insulin release: evidence for specifity of impaired beta cell adaptation. Diabetologia, 48, 1350–1358.

    Article  CAS  PubMed  Google Scholar 

  • Jensen, C., Cnop, M., Hull, R. L., Fujimoto, W. Y., Kahn, S. E. and the American Diabetes Association GENNID Study Group, 2002. Cell function is a major contributor to oral glucose tolerance in high-risk relatives of four ethnic groups in the US. Diabetes, 51, 2170–2178.

    Article  CAS  PubMed  Google Scholar 

  • Kahn, St. E., 2001. The importance of β-cell failure in development and progression of type 2 diabetes. The Journal of Clinical Endocrinology metabolism, 86, 4047–4058.

    Article  CAS  Google Scholar 

  • Kahn, S. E., 2003. The relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of type 2 diabetes. Diabetologia, 46, 3–19.

    Article  CAS  PubMed  Google Scholar 

  • Kahn, S.E., Montgomery, B., Howell, W, Ligueros-Saylan, M., Hsu, C.H., Devineni, D., McLeod, J. F, Horowitz, A. and J.E. Foley, 2001. Importance of early phase insulin secretion to intravenous glucose tolerance in subjects with type 2 diabetes mellitus. Journal of Clinical Endocrinology and Metabolism, 86, 5824–5829.

    Article  CAS  PubMed  Google Scholar 

  • Kaiyala, K. J., Prigeon, R. L., Kahn, S. E., Woods, S. C., Porte, D. J., Schwartz, M. W., 1999. Reduced beta-cell function contributes to impaired glucose tolerance in dogs made obese by high-fat feeding. American Journal of Physiology, 126, 123–131.

    Google Scholar 

  • Kern, Ph. A., Ranganathan, S., Li, Ch., Wood L., Ranganathan, G., 2001. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. American Journal of Physiology—Endocrinology and Metabolism, 280, 745–E751.

    Google Scholar 

  • Kharroubi, I.L., Ladriere, L., Cardozo, A. K., Dogusan, Z., Cnop, M., Eizirik, D. L., 2004. Free fatty acids and cytokines induce pancreatic β-cell apoptosis by different mechanisms: role of nuclear factor—kB and endoplasmic reticulum stress. Endocrinology, 145, 11 5087–5096.

    Article  CAS  PubMed  Google Scholar 

  • Kim, S. P., Ellmerer, M., Bergman, R.N., 2003. Primacy of hepatic insulin resistance in the development of the metabolic syndrome induced by an isocaloric moderate-fat diet in the dog. Diabetes, 52 (10), 2453–60.

    Article  CAS  PubMed  Google Scholar 

  • Larsson, H. ,Ahren B., 1996. Failure to adequately adapt reduced insulin sensitivity with increased insulin secretion in women with impaired glucose tolerance. Diabetologia, 39, 1099–1107.

    Article  CAS  PubMed  Google Scholar 

  • Mattheeuws, D., Rottiers, R., Kaneko, J.J. ,Vermeulen, A., 1984. Diabetes mellitus in dogs: relationship of obesity to glucose tolerance and insulin response. American Journal of Veterinary Research, 45, 98–103.

    CAS  PubMed  Google Scholar 

  • Mattheeuws, D., Rottiers, R., Baeyens, D, Vermeulen, A., 1982. Glucose tolerance and insulin response in obese dog. Journal of American Animal Hospital Association, 20, 287–293.

    Google Scholar 

  • McGuinness, O., Donmoyer, C., Ejiofor, J., McElligott, S., Brooks Lacy, D., 1998. Hepatic and muscles glucose metabolism during total parenteral nutrition: impact of infection. American Journal of Physiology, 275, E763–E769.

    CAS  PubMed  Google Scholar 

  • Mittelman, S. D., Van-Citters, G. W., Kirkman, E. L., Bergman, R. N., 2002. Extreme insulin resistance of the central adipose depot in vivo. Diabetes, 51, 755–761.

    Article  CAS  PubMed  Google Scholar 

  • Newgard, C.B., McGarry, J.D., 1995. Metabolic coupling factors in pancreatic ß-cell signal transduction. Annual Review of Biochemistry, 64, 689–719.

    Article  CAS  PubMed  Google Scholar 

  • Odegaard, J. I., Ricardo-Gonzalez, R. R., Goforth, M. H., Morel C. R., Subramanian, V., Mukundan L., Eagle, A. R.,Vats, D., Brombacher, F., Ferrante, A. W. and Chawla, A., 2007. Macrophage-specific PPAR-γ controls alternative activation and improves insulin resistance. Nature, 477, 1116–1120.

    Article  Google Scholar 

  • Pitout, V. and Robertson, R. P., 2008. Glucolipotoxicity: Fuel excess and β-cell dysfunction. Endocrine Reviews, 29, 351–366.

    Article  Google Scholar 

  • Porte, D., 1991. Cells in diabetes mellitus. Diabetes, 40, 166–180.

    Article  PubMed  Google Scholar 

  • Rand, J. S., Fleeman, L. M., Farrow, H. A., Appleton, D. J., and Lederer, R., 2004. Canine and feline diabetes mellitus: nature or nurture? Journal of Nutrition, 134, 2072S–2080S.

    CAS  PubMed  Google Scholar 

  • Rocchini, A. P., Mao, H. Z., Babu, K., Marker, P., Rocchini, A. J., 1999. Clonidine prevents insulin resistance and hypertension in obese dogs. Hypertension, 33, 548–553.

    CAS  PubMed  Google Scholar 

  • Sammalkorpi, K., 2007. Glucose tolerance in acute infections. Journal of Internal Medicine, 225, 15–19.

    Article  Google Scholar 

  • Schwartz, M. W. and Kahn, S. E., 1999. Diabetes: insulin resistance and obesity. Nature, 420, 860–861.

    Article  Google Scholar 

  • Stienstra, R., Duval, C., Muller, M., 2007. PPARs, obesity and inflammation. PPAR Researc, 2007, 95974.

    Google Scholar 

  • Stumvoll, M., 2007. Metabolic syndrome in humans. Proceedings 13th International Conferance on Production Diseases in Farm Animals. M. Furll (ed.) Leipzig.

  • Stumvoll, M., Goldstein, B., van Haefen T., 2005a. Type 2 diabetes: principales and therapy. Lancet, 365, 1333–1346.

    Article  CAS  Google Scholar 

  • Stumvoll, M., Tataranni, P.A., Bogardus, C., 2005b. The huperbolic law—a 25-year perspective. Diabetologia, 48, 207–209.

    Article  CAS  Google Scholar 

  • Swinburn, B. A., Boyce, V. L., Bergman, R. N., Howard, B. V., Bogardus C., 1991. Deterioration in carbohydrate metabolism and lipoprotein changes induced by modern, high fat diet in Pima Indians and Caucasians. Journal of Clinical Endocrinology and Metabolism, 73, 156–165.

    Article  CAS  PubMed  Google Scholar 

  • Thiess, S., Becskei, C., Tomsa, K., Lutz, T. A. and Wanner, M., 2004. Effects of high carbohydrate and high fat diet on plasma metabolite levels and on iv glucose tolerance test in intact and neutered male cats. Journal of Feline Medicine and Surgery, 6, 207–218.

    Article  CAS  PubMed  Google Scholar 

  • Truett, A. A., Borne, A. T., Monteiro, M. P. & West, D. B., 1998. Composition of dietary fat affects blood pressure and insulin responses to dietary obesity in the dog. Obesity Research, 6, 137–146.

    CAS  PubMed  Google Scholar 

  • Villa, E., Gonzalez-Albarran, O., Rabano, A., Garcia-Robles, R., 1999. Effects of hyperinsulinemia on vascular blood flows in experimental obesity. Journal of Steroid Biochemistry and Molecular Biology, 69, 273–279.

    Article  CAS  PubMed  Google Scholar 

  • Weyer,C., Tataranni P.A., 2001. Insulin resistance and insulin secretoty dysfunction are independent predictors of worsening of glucose tolerance during each stage of type 2 diabetes development. Diabetes care, 24, № 1.

    Google Scholar 

  • Williams, K., Hanley, A. J. G., Haffner, S. M., Stern, M. P., 2003. Predicting diabetes with insulin secretion measures in the San Antonio Heart Study. Diabetes and Metabolism, 29, 5S20–S31.

    Google Scholar 

  • You, T., Yang, R., Lyles, M., Gong, D., Nicklas B.J., 2005. Abdominal adipose tissue cytokine gene expression: relationship to obesity and metabolic risk factors. American Journal of Physiololgy—Endocrinology and Metabolisme, 288, E741–E747.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Pathology, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria

    Evgeni Slavov, Petko Dzhelebov & Maria Andonova

  2. Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria

    Ivan Penchev Georgiev, Ivan Kanelov, Teodora Mircheva Georgieva & Silviya Dimitrova

Authors
  1. Evgeni Slavov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ivan Penchev Georgiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Petko Dzhelebov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ivan Kanelov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maria Andonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Teodora Mircheva Georgieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Silviya Dimitrova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ivan Penchev Georgiev.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Slavov, E., Georgiev, I.P., Dzhelebov, P. et al. High-fat feeding and staphylococcus intermedius infection impair beta cell function and insulin sensitivity in mongrel dogs. Vet Res Commun 34, 205–215 (2010). https://doi.org/10.1007/s11259-010-9345-x

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11259-010-9345-x

Keywords

Search

Navigation