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Genipin Inhibits LPS-Induced Inflammatory Response in BV2 Microglial Cells

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Abstract

Genipin, an aglycon of geniposide, has been reported to have anti-inflammatory effect. However, the anti-inflammatory activity of genipin on LPS-stimulated BV2 microglial cells has not been reported. In this study, we investigated the molecular mechanisms responsible for the anti-inflammatory activity of genipin both in vivo and in vitro. The levels of TNF-α, IL-1β, NO and PGE2 were detected by ELISA. The expression of Nrf2, HO-1, and NF-κB were detected by western blot analysis. In vivo, genipin significantly attenuated LPS-induced memory deficit in the Morris water maze and passive avoidance tasks. Genipin also inhibited LPS-induced TNF-α and IL-1β expression in brain tissues. In vitro, our results showed that genipin inhibited LPS-induced TNF-α, IL-1β, NO and PGE2 production in a concentration-dependent manner. Genipin also suppressed LPS-induced NF-κB activation. In addition, the expression of Nrf2 and HO-1 were up-regulated by treatment of genipin. Furthermore, the inhibition of genipin on inflammatory mediator production was attenuated by transfection with Nrf2 siRNA. In conclusion, genipin inhibited LPS-induced inflammatory response by activating Nrf2 signaling pathway in BV2 microglia.

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Acknowledgements

This work was supported by grants from First prize of China Postdoctoral Science Foundation; Doctoral Fund of The second affiliated to Harbin Medical University; Foundation for Returness of Ministry of Education of China. This Study was supported by the Grant from Heilongjiang returned overseas science fund project (No. LC2013C40), Chinese Postdoctoral Science Foundation (No. 2014M561373), and Heilongjiang Postdoctoral Science Foundation (No. LBH-Z14144).

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    1. Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin, 150086, China

      Jianjiao Wang, Liang Chen, Zuobin Liang, Yang Li, Fei Yuan, Jie Liu, Yu Tian, Zhen Hao, Fucheng Zhou, Xuanxi Liu, Yu Cao, Yongri Zheng & Qingsong Li

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    1. Jianjiao Wang
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    Jianjiao Wang and Liang Chen have contributed equally to this article.

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    Wang, J., Chen, L., Liang, Z. et al. Genipin Inhibits LPS-Induced Inflammatory Response in BV2 Microglial Cells. Neurochem Res 42, 2769–2776 (2017). https://doi.org/10.1007/s11064-017-2289-6

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