Abstract
The pathogenesis of stroke is associated with the immune and inflammatory responses. Cytokines, such as interleukin 10 (IL10), play an important role in the process of inflammation. To investigate whether IL10, IL10RA, and IL10RB polymorphisms are associated with the risk of ischemic stroke (IS), selected two IL10 SNPs (rs1518111 and rs1554286), three IL10RA SNPs (rs2256111, rs4252243, and rs2228054), and two IL10RB SNPs (rs999788 and rs2834167) were analyzed in 120 patients with IS and 285 control subjects. All IS patients were classified into the clinical subgroups, according to the levels of blood pressure (hypertension, present and absent), fasting plasma glucose (diabetes mellitus, present and absent), and lipids (dyslipidemia, present and absent). SNPStats and SPSS 18.0 program were used to obtain the odds ratios, 95 % confidence intervals, and P values. Multiple logistic regression models (codominant1, codominant2, dominant, recessive, and log-additive models) were performed to analyze the genetic data. Seven polymorphisms were not associated with the IS, but showed significant associations with hypertension, in the risk of IS. These results suggest that the IL10, IL10RA, and IL10RB genes may be contributed to the hypertension in the risk of IS in the Korean population.
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References
Dichgans M (2007) Genetics of ischaemic stroke. Lancet Neurol 6:149–161
Balding J, Livingstone WJ, Pittock SJ, Mynett-Johnson L, Ahern T, Hodgson A, Smith OP (2004) The il-6g-174c polymorphism may be associated with ischaemic stroke in patients without a history of hypertension. Ir J Med Sci 173:200–203
Kim Y, Lee C (2006) The gene encoding transforming growth factor beta 1 confers risk of ischemic stroke and vascular dementia. Stroke 37:2843–2845
Li N, He Z, Xu J, Liu F, Deng S, Zhang H (2009) Association of pde4d and il-1 gene polymorphism with ischemic stroke in a han chinese population. Brain Res Bull 81:38–42
Marousi S, Ellul J, Antonacopoulou A, Gogos C, Papathanasopoulos P, Karakantza M (2010) Functional polymorphisms of interleukin 4 and interleukin 10 may predict evolution and functional outcome of an ischaemic stroke. Eur J Neurol 18:637–643
Sultana S, Kolla VK, Jeedigunta Y, Penagaluru PK, Joshi S, Rani PU, Reddy PP (2011) Tumour necrosis factor alpha and interleukin 10 gene polymorphisms and the risk of ischemic stroke in south indian population. J Genet 90:361–364
Koch W, Kastrati A, Bottiger C, Mehilli J, von Beckerath N, Schomig A (2001) Interleukin-10 and tumor necrosis factor gene polymorphisms and risk of coronary artery disease and myocardial infarction. Atherosclerosis 159:137–144
Munshi A, Rajeshwar K, Kaul S, Al-Hazzani A, Alshatwi AA, Sai Babu M, Usha A, Jyothy A (2010) Interleukin-10-1082 promoter polymorphism and ischemic stroke risk in a south Indian population. Cytokine 52:221–224
Yoo KH, Kim SK, Chung JH, Chang SG (2011) Association of il10, il10ra, and il10rb polymorphisms with benign prostate hyperplasia in Korean population. J Korean Med Sci 26:659–664
Lewis CM (2002) Genetic association studies: design, analysis and interpretation. Brief Bioinform 3:146–153
Barrett JC, Fry B, Maller J, Daly MJ (2005) Haploview: analysis and visualization of ld and haplotype maps. Bioinformatics 21:263–265
Herz J, Reitmeir R, Hagen SI et al (2011) Intracerebroventricularly delivered vegf promotes contralesional corticorubral plasticity after focal cerebral ischemia via mechanisms involving anti-inflammatory actions. Neurobiol Dis 45:1077–1085
Peng Z, Zhan L, Chen S, Xu E (2011) Association of transforming growth factor-beta1 gene c-509t and t869c polymorphisms with atherosclerotic cerebral infarction in the Chinese: a case-control study. Lipids Health Dis 10:100
Lee BC, Ahn SY, Doo HK et al (2004) Susceptibility for ischemic stroke in Korean population is associated with polymorphisms of the interleukin-1 receptor antagonist and tumor necrosis factor-alpha genes, but not the interleukin-1beta gene. Neurosci Lett 357:33–36
Marousi S, Antonacopoulou A, Kalofonos H, Papathanasopoulos P, Karakantza M, Ellul J (2011) Functional inflammatory genotypes in ischemic stroke: could we use them to predict age of onset and long-term outcome? Stroke Res Treat 2011:792923
Moore KW, O’Garra A, de Waal Malefyt R, Vieira P, Mosmann TR (1993) Interleukin-10. Annu Rev Immunol 11:165–190
Girndt M, Kohler H (2003) Interleukin-10 (il-10): an update on its relevance for cardiovascular risk. Nephrol Dial Transplant 18:1976–1979
Jian Liu K, Rosenberg GA (2005) Matrix metalloproteinases and free radicals in cerebral ischemia. Free Radic Biol Med 39:71–80
Eskdale J, Keijsers V, Huizinga T, Gallagher G (1999) Microsatellite alleles and single nucleotide polymorphisms (snp) combine to form four major haplotype families at the human interleukin-10 (il-10) locus. Genes Immun 1:151–155
Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV (1997) An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24:1–8
Timasheva YR, Nasibullin TR, Zakirova AN, Mustafina OE (2008) Association of interleukin-6, interleukin-12, and interleukin-10 gene polymorphisms with essential hypertension in tatars from Russia. Biochem Genet 46:64–74
Kalkunte S, Nevers T, Norris WE, Sharma S (2011) Vascular il-10: a protective role in preeclampsia. J Reprod Immunol 88:165–169
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The present research was conducted by the research fund of Dankook University in 2011.
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Park, HK., Kim, D.H., Yun, D.H. et al. Association between IL10, IL10RA, and IL10RB SNPs and ischemic stroke with hypertension in Korean population. Mol Biol Rep 40, 1785–1790 (2013). https://doi.org/10.1007/s11033-012-2232-5
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DOI: https://doi.org/10.1007/s11033-012-2232-5