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The protective effect of quercetin on long-term alcohol consumption-induced oxidative stress

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Abstract

Long-term alcohol consumption can cause oxidative stress and cytokines induction, which are associated with free radicals. Quercetin, one of the most widely distributed flavonoids in plants, is a natural antioxidant. We investigated the hypothesis that quercetin could prevent the ethanol-induced oxidative stress and decreases tumor necrosis factor-α (TNF-α) and interferon-γ (INF-γ) as pro-inflammatory cytokines. Twenty-eight rats were randomly divided into control group (C), ethanol treatment group (EtOH) (~1 ml/day, 80%; 2 g/kg body wt), intragastrically (i.g.), quercetin treatment group (Q), (100 mg/kg-body wt per 3 days) i.g. and ethanol plus quercetin treatment group (EtOH + Q) (1 ml/day, 80% of ethanol and 100 mg/kg-body wt of quercetin per 3 days) i.g. for 30 days Plasma thiobarbituric acid reactive substance (TBARS) levels and protein carbonyl content were significantly higher in the EtOH group than the C group (P < 0.01). On the other hand, TBARS level and protein carbonyl content in the EtOH + Q group was decreased significantly by quercetin (P < 0.05, P < 0.01; respectively). While GSH levels in whole blood decreased in EtOH group compared to C group, they increased significantly by quercetin (P < 0.05). Plasma ALT, TNF-α and IFN-γ levels increased significantly in the EtOH group compared to control group (P < 0.05, P < 0.01, P < 0.01, respectively), but they decreased significantly in the EtOH + Q group in comparison with EtOH group (P < 0.05, P < 0.01, P < 0.01, respectively). Our results demonstrate that quercetin treatment may provide a protection as reflected by decreased plasma TBARS, protein carbonyls, TNF-α, INF-γ and ALT levels against ethanol-induced oxidative damage.

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References

  1. Liu S, Hou W, Yao P et al (2010) Quercetin protects against ethanol-induced oxidative damage in rat primary hepatocytes. Toxicol In Vitro 24(2):516–522. Epub 2009 Mar 25

    Google Scholar 

  2. Molina MF, Sanchez-Reus I, Iglesias I, Benedi J (2003) Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biol Pharm Bull 26(10):1398–1402

    Article  PubMed  CAS  Google Scholar 

  3. Zhou Z, Wang L, Song Z, Lambert JC, McClain CJ, Kang YJ (2003) A critical involvement of oxidative stress in acute alcohol-induced hepatic TNF-alpha production. Am J Pathol 163(3):1137–1146

    Article  PubMed  CAS  Google Scholar 

  4. Zhou Z, Wang L, Song Z et al (2005) Zinc supplementation prevents alcoholic liver injury in mice through attenuation of oxidative stress. Am J Pathol 166(6):1681–1690

    Article  PubMed  CAS  Google Scholar 

  5. Lieber CS (1997) Alcohol metabolism, chrosis and alcoholism. Clin Chim Acta 257(1):59–84

    Article  PubMed  CAS  Google Scholar 

  6. Tyulina OV, Prokopieva VD, Dodd RD et al (2002) In vitro effects of ethanol, acetaldehyde and fatty acid ethyl esters on human erythrocytes. Alcohol Alcohol 37(2):179–186

    PubMed  CAS  Google Scholar 

  7. Oh SI, Kim CI, Chun HJ, Park SC (1998) Chronic ethanol consumption affects glutathione status in rat liver. J Nutr 128(4):758–763

    PubMed  CAS  Google Scholar 

  8. Singh A, Naidu PS, Kulkarni SK (2003) Reversal of aging and chronic ethanol-induced cognitive dysfunction by quercetin a bioflavonoid. Free Radic Res 37(11):1245–1252

    Article  PubMed  CAS  Google Scholar 

  9. Purohit V, Russo D, Salin M (2003) Role of iron in alcoholic liver disease: introduction and summary of the symposium. Alcohol 30(2):93–97

    Article  PubMed  Google Scholar 

  10. Kahraman A, Erkasap N, Köken T et al (2003) The antioxidative and antihistaminic properties of quercetin in ethanol-induced gastric lesions. Toxicology 183(1–3):133–142

    Article  PubMed  CAS  Google Scholar 

  11. Oh SI, Kim CI, Chun HJ, Lee MS, Park SC (1997) Glutathione recycling is attenuated by acute ethanol feeding in rat liver. J Korean Med Sci 12(4):316–321

    PubMed  CAS  Google Scholar 

  12. Kessova IG, Ho YS, Thung S, Cederbaum AI (2003) Alcohol-induced liver injury in mice lacking Cu, Zn-superoxide dismutase. Hepatology 38(5):1136–1145

    Article  PubMed  CAS  Google Scholar 

  13. Zhou Z, Liu J, Song Z, McClain CJ, Kang YJ (2008) Zinc supplementation inhibits hepatic apoptosis in mice subjected to a long-term ethanol exposure. Exp Biol Med (Maywood) 233(5):540–548. Epub 2008 Mar 28

    Google Scholar 

  14. Liu JL, Du J, Fan LL et al (2008) Effects of quercetin on hyper-proliferation of gastric mucosal cells in rats treated with chronic oral ethanol through the reactive oxygen species-nitric oxide pathway. World J Gastroenterol 14(20):3242–3248

    Article  PubMed  CAS  Google Scholar 

  15. McClain CJ, Shedlofsky S, Barve S, Hill DB (1997) Cytokines and alcoholic liver disease. Alcohol Health Res World 21(4):317–320

    PubMed  CAS  Google Scholar 

  16. Andrade MC, Martins-Filho OA, Coelho-Neto J, Mesquita ON, Faria AM (2008) The long-term impaired macrophages functions are already observed early after high-dose ethanol administration. Scand J Immunol 68(3):306–314

    Article  PubMed  CAS  Google Scholar 

  17. Naveau S, Abella A, Raynard B et al (2001) Tumor necrosis factor soluble receptor p55 and lipid peroxidation in patients with acute alcoholic hepatitis. Am J Gastroenterol 96(12):3361–3367

    Article  PubMed  CAS  Google Scholar 

  18. Robin MA, Demeilliers C, Sutton A et al (2005) Alcohol increases tumor necrosis factor alpha and decreases nuclear factor-kappa b to activate hepatic apoptosis in genetically obese mice. Hepatology 42(6):1280–1290

    Article  PubMed  CAS  Google Scholar 

  19. İnal ME, Kahraman A (2000) The protective effect of flavonol quercetin against ultraviolet a induced oxidative stress in rats. Toxicology 154(1–3):21–29

    Google Scholar 

  20. Mizui T, Sato H, Hirose F, Doteuchi M (1987) Effect of antiperoxidative drugs on damage induced by ethanol in rats. Life Sci 41(6):755–763

    Article  PubMed  CAS  Google Scholar 

  21. Wu CH, Wu CF, Huang HW, Jao YC, Yen GC (2009) Naturally occurring flavonoids attenuate high glucose-induced expression of proinflammatory cytokines in human monocytic THP-1 cells. Mol Nutr Food Res 53(8):984–995

    Article  PubMed  CAS  Google Scholar 

  22. De Stefano D, Maiuri MC, Simeon V et al (2007) Lycopene, quercetin and tyrosol prevent macrophage activation induced by gliadin and IFN-gamma. Eur J Pharmacol 566(1–3):192–199. Epub 2007 Apr 6

    Google Scholar 

  23. Ohkawa H, Ohishi N, Yagi K (1979) Assay for Lipid peroxidase in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358

    Article  PubMed  CAS  Google Scholar 

  24. Levine RL, Garland D, Oliver CN (1990) Determination of carbonyl content in oxidatively modified proteins. Method Enzymol 186:464–478

    Article  CAS  Google Scholar 

  25. Beutler E (1957) The Glutathione instability of drug sensitivity red cells. J Lab Clin Med 49(1):84–95

    PubMed  CAS  Google Scholar 

  26. Trnovsky J, Letourneau R, Haggag E, Boucher W, Theoharides TC (1993) Quercetin induced expression of rat mast cell protease II and accumulation of secretory granules in rat basophilic leukemia cells. Biochem. Pharmacol 46(12):2315–2326

    CAS  Google Scholar 

  27. Takahama U (1985) Inhibition of lipoxygenase-dependent lipid peroxidation by quercetin: mechanism of antioxidative function. Phytochemistry 24(7):1443–1446

    Article  CAS  Google Scholar 

  28. Hernández-Muñoz R, Montiel-Ruíz C, Vázquez-Martínez O (2000) Gastric mucosal cell proliferation in ethanol-induced chronic mucosal injury is related to oxidative stress and lipid peroxidation in rats. Lab Invest 80(8):1161–1169

    Article  PubMed  Google Scholar 

  29. Luczaj W, Siemieniuk E, Roszkowska-Jakimiec W, Skrzydlewska E (2006) Protective effect of black tea against ethanol-induced oxidative modifications of liver proteins and lipids. J Stud Alcohol 67(4):510–518

    PubMed  Google Scholar 

  30. Reddy VD, Padmavathi P, NCh Varadacharyulu (2009) Emblica officinalis protects against alcohol-induced liver mitochondrial dysfunction in rats. J Med Food 12(2):327–333

    Article  PubMed  CAS  Google Scholar 

  31. Abraham P, Wilfred G, Ramakrishna B (2002) Oxidative damage to the hepatocellular proteins after chronic ethanol intake in the rat. Clin Chim Acta 325(1–2):117–125

    Article  PubMed  CAS  Google Scholar 

  32. Yao P, Nussler A, Liu L, et al (2007) Quercetin protects human hepatocytes from ethanol-derived oxidative stress by inducing heme oxygenase-1 via the MAPK/Nrf2 pathways. J Hepatol 47(2):253–261. Epub 2007 Mar 7

    Google Scholar 

  33. Albano E, Vidali (2009) M Immune mechanisms in alcoholic liver disease. Genes Nutr 5(2):141–147

    Article  Google Scholar 

  34. Gustot T, Lemmers A, Moreno C et al (2006) Differential liver sensitization to toll-like receptor pathway in mice with alcoholic fatty liver. Hepatology 43(5):989–1000

    Article  PubMed  CAS  Google Scholar 

  35. Kahraman A, İnal ME (2002) Protective effects of quercetin on ultraviolet A light-induced oxidative stress in the blood of rat. J Appl Toxicol 22(5):303–309

    Article  PubMed  CAS  Google Scholar 

  36. Tokyol C, Yilmaz S, Kahraman A, Cakar H, Polat C (2006) The effects of desferrioxamine and quercetin on liver injury induced by hepatic ischaemia-reperfusion in rats. Acta Chir Belg 106(1):68–72

    PubMed  CAS  Google Scholar 

  37. Kono H, Arteel GE, Rusyn I, Sies H, Thurman RG (2001) Ebselen prevents early alcohol-induced liver injury in rats. Free Radic Biol Med 30(4):403–411

    Article  PubMed  CAS  Google Scholar 

  38. Kawada N, Seki S, Inoue M, Kuroki T (1998) Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells. Hepatology 27(5):1265–1274

    Article  PubMed  CAS  Google Scholar 

  39. Yu ES, Min HJ, An SY et al (2008) Regulatory mechanisms of IL-2 and IFN-gamma suppression by quercetin in T helper cells. Biochem Pharmacol 76(1):70–78. Epub 2008 Apr 6

    Google Scholar 

  40. Park HH, Lee S, Son HY et al (2008) Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Arch Pharm Res 31(10):1303–1311. Epub 2008 Oct 29

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, 03200, Turkey

    Ahmet Kahraman, Hamdullah Çakar & Tülay Köken

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  1. Ahmet Kahraman
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  2. Hamdullah Çakar
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  3. Tülay Köken
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Correspondence to Ahmet Kahraman.

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Kahraman, A., Çakar, H. & Köken, T. The protective effect of quercetin on long-term alcohol consumption-induced oxidative stress. Mol Biol Rep 39, 2789–2794 (2012). https://doi.org/10.1007/s11033-011-1037-2

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  • DOI: https://doi.org/10.1007/s11033-011-1037-2

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