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Association study of four variants in KCNQ1 with type 2 diabetes mellitus and premature coronary artery disease in a Chinese population

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Abstract

Four single nucleotide polymorphisms (SNPs, rs2237892, rs2237895, rs2237897, rs2283228) in KCNQ1 are associated with type 2 diabetes mellitus in different ancestral groups. We investigated whether these 4 genetic markers are determinants of type 2 diabetes and premature coronary artery disease (CAD) in a Chinese population. We studied 398 consecutive patients, including 180 with coronary stenosis ≥50% or previous myocardial infarction (male <55 years, female <65 years) and 218 controls without documented CAD. CAD cases and controls were genotyped for 4 SNPs by using the ligase detection reaction method. The 3 genotypes AA, AC, and CC were present in rs2283228 and rs2237895, and the 3 genotypes CC, CT, TT were present in rs2237897 and rs2237892. No differences were found in genotype distribution and allele frequencies of these 4 SNPs between subjects with and without type 2 diabetes. Logistic regression showed that the risk of premature CAD in subjects carrying the CC genotype at rs2237892 was reduced by 90% in relation to individuals carrying the TT genotype (OR = 0.100, 95% CI: 0.018–0.564, P = 0.009). The association of other 3 SNPs with premature CAD could not be detected, nor did there exist any association of these 4 SNPs among groups of patients with 0, 1, 2, and 3-vessel disease (all P > 0.05). Our data implicate rs2237892 in KCNQ1 as a protective gene variant against premature CAD and we couldn’t replicate any association of these 4 SNPs with T2DM or extent of coronary lesions in a Chinese population.

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Acknowledgments

This study was supported by grants from the Ministry of Education of P. R. China and the Nanjing Scientific Development Project (2006ZD016).

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Authors and Affiliations

  1. Department of Cardiology, The Affiliated ZhongDa Hospital of Southeast University, NO.87 Dingjiaqiao, Hunan Road, 210009, Nanjing, People’s Republic of China

    Zhong Chen, Xiaofeng Zhang, Genshan Ma, Qi Qian & Yuyu Yao

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  1. Zhong Chen
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  2. Xiaofeng Zhang
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  3. Genshan Ma
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  4. Qi Qian
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  5. Yuyu Yao
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Correspondence to Zhong Chen.

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Chen, Z., Zhang, X., Ma, G. et al. Association study of four variants in KCNQ1 with type 2 diabetes mellitus and premature coronary artery disease in a Chinese population. Mol Biol Rep 37, 207–212 (2010). https://doi.org/10.1007/s11033-009-9597-0

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  • DOI: https://doi.org/10.1007/s11033-009-9597-0

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