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dsRNA-mediated resistance to Beet Necrotic Yellow Vein Virus infections in sugar beet (Beta vulgaris L. ssp. vulgaris)

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Abstract

Rhizomania, one of the most devastating diseases in sugar beet, is caused by Beet Necrotic Yellow Vein Virus (BNYVV) belonging to the genus Benyvirus. Use of sugar beet varieties with resistance to BNYVV is generally considered as the only way to maintain a profitable yield on rhizomania-infested fields. As an alternative to natural resistance, we explored the transgenic expression of viral dsRNA for engineering resistance to rhizomania. Transgenic plants expressing an inverted repeat of a 0.4 kb fragment derived from the BNYVV replicase gene displayed high levels of resistance against different genetic strains of BNYVV when inoculated using the natural vector, Polymyxa betae. The resistance was maintained under high infection pressures and over prolonged growing periods in the greenhouse as well as in the field. Resistant plants accumulated extremely low amounts of transgene mRNA and high amounts of the corresponding siRNA in the roots, illustrative of RNA silencing as the underlying mechanism. The transgenic resistance compared very favourably to natural sources of resistance to rhizomania and thus offers an attractive alternative for breeding resistant sugar beet varieties.

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Acknowledgements

The authors wish to thank Nathalie Sauviac, Morten Joersbo, Kirsten Jörgensen, Susann Lund, Heléne Sandell, Gun Karlsson and Gabriella Arndt for skilful assistance and Maria Nihlgård, Leif Johansson, Flemming Yndgaard and Thomas Kraft for fruitful discussions.

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Authors and Affiliations

  1. Syngenta Seeds AB, 302, SE-261 23, Landskrona, Sweden

    Britt-Louise Lennefors, Jan Bensefelt, Elisabeth Wremerth-Weich, Petra van Roggen & Stig Tuvesson

  2. Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 7080, SE-750 07, Uppsala, Sweden

    Eugene I. Savenkov & Jari P. T. Valkonen

  3. Department of Applied Biology, University of Helsinki, 27, FIN-00014, Helsinki, Finland

    Jari P. T. Valkonen

  4. Syngenta Seeds, 12 chemin de l’hobit, 31790, Saint-Sauveur, France

    Jan Gielen

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  1. Britt-Louise Lennefors
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  2. Eugene I. Savenkov
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  3. Jan Bensefelt
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  4. Elisabeth Wremerth-Weich
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  5. Petra van Roggen
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  6. Stig Tuvesson
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  7. Jari P. T. Valkonen
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  8. Jan Gielen
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Correspondence to Britt-Louise Lennefors.

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Lennefors, BL., Savenkov, E., Bensefelt, J. et al. dsRNA-mediated resistance to Beet Necrotic Yellow Vein Virus infections in sugar beet (Beta vulgaris L. ssp. vulgaris). Mol Breeding 18, 313–325 (2006). https://doi.org/10.1007/s11032-006-9030-5

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  • DOI: https://doi.org/10.1007/s11032-006-9030-5

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