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Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague–Dawley rats

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Abstract

The present study evaluated the effects of asiatic acid (AA), a pentacyclic triterpenoid from Centella asiatica on lipid metabolism parameters in a rat model of obesity induced using a high fat diet (HFD) for 42 days. AA (20 mg/kg body weight [BW]) was administered orally once daily for 42 days, and an orlistat-treated group of rats (10 mg/kg BW) was included for comparison. Changes in BW, blood glucose levels, insulin resistance and leptin, adiponectin, amylase, and lipase levels in the blood; lipid profiles of plasma; liver antioxidants levels; and acetyl CoA carboxylase(ACC), uncoupling protein-2 (UCP2), and carnitine palmitoyltransferase-1 (CPT1) mRNA expression were observed in the experimental rats. Our results revealed that AA (20 mg/kg BW), similar to orlistat, reduced the increase in BW; increased bone mineral contents and bone mineral densities; reduced blood glucose levels, insulin resistance, leptin, plasma lipid levels; increased adiponectin, amylase, lipase levels in the blood; showed antioxidant activity; and altered mRNA expression of lipid metabolism-related genes, including ACC, UCP 2, and CPT 1, in the HFD-fed rats. From these results, we concluded that AA possesses significant anti-obesity potential through the suppression of BW gain, lipid lowering action, development of insulin and leptin sensitivity, antioxidant activity, and increased mRNA expression of lipid metabolism-related genes.

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Acknowledgements

The authors thank Muthyammal College of Arts and Science, Rasipuram, Tamilnadu, India, for providing facilities to do animal studies and also express heartfelt thanks to the management of K. S. Rangasamy College of Arts and Science, Tiruchengode, Tamilnadu, for their support and encouragement.

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Author notes

  1. P. Rameshreddy, V. V. Sathibabu Uddandrao, and Parim Brahmanaidu have contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry, Centre for Biological Sciences, K. S. Rangasamy College of Arts and Science (Autonomous), Namakkal Dt, Tiruchengode, Tamil Nadu, 637215, India

    P. Rameshreddy, V. V. Sathibabu Uddandrao, S. Vadivukkarasi, K. Swapna, A. Kalaivani & Ganapathy Saravanan

  2. Department of Biotechnology, Vikrama Simhapuri University, Nellore, Andhra Pradesh, 524003, India

    Parim Brahmanaidu & Parimi Parvathi

  3. National Centre for Laboratory Animal Sciences, National Institute of Nutrition, Hyderabad, 500007, India

    Ramavat Ravindarnaik & Pothani Suresh

  4. Department of Biochemistry, PGP College of Art and Science, Namakkal, Tamil Nadu, 637207, India

    A. Kalaivani & P. Tamilmani

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  1. P. Rameshreddy
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Correspondence to Ganapathy Saravanan.

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All procedures involving laboratory animals were in accordance with the guidelines of Committee for the purpose of control and supervision of experiments on animals (CPCSEA), Government of India, and institutional animal ethical committee of Muthyammal College of Arts and Science (Approval No: IAEC/MCAS/05/2016).

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Rameshreddy, P., Uddandrao, V.V.S., Brahmanaidu, P. et al. Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague–Dawley rats. Mol Cell Biochem 442, 143–154 (2018). https://doi.org/10.1007/s11010-017-3199-2

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