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miR-3117 regulates hepatocellular carcinoma cell proliferation by targeting PHLPPL

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Abstract

Altered microRNA expression is associated with tumor proliferation, metastasis, and tumorigenesis. In this study, we studied the role of miR-3117 in hepatocellular carcinoma (HCC) cell proliferation and found that miR-3117 was upregulated in HCC tissues and cells. MTT assay, soft agar growth assay, BrdU assay, and cell cycle assay revealed that miR-3117 overexpression promoted HCC HepG2 cell proliferation and that knockdown of miR-3117 suppressed HepG2 proliferation. Mechanism analysis suggested PH domain and leucine-rich repeat protein phosphatase-like (PHLPPL) as the target of miR-3117. Luciferase reporter assay suggested that miR-3117 directly binds to the 3′UTR of PHLPPL. Double knockdown of miR-3117 and PHLPPL copied the phenotypes caused by miR-3117 overexpression, suggesting that miR-3117 contributes to the proliferation of HepG2 by targeting PHLPPL. Our study provided a target for HCC therapy.

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    Authors and Affiliations

    1. Department of Hepatopathy, Liaocheng People’s Hospital, 67 Dongchang West Road, Liaocheng, 252000, China

      Xia Cui, Qingyan Li & Yukai He

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    1. Xia Cui
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    2. Qingyan Li
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    3. Yukai He
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    Correspondence to Yukai He.

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    Xia Cui and Qingyan Li contributed equally to this work.

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    Cui, X., Li, Q. & He, Y. miR-3117 regulates hepatocellular carcinoma cell proliferation by targeting PHLPPL. Mol Cell Biochem 424, 195–201 (2017). https://doi.org/10.1007/s11010-016-2855-2

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