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The effect of combined regulation of the expression of peroxisome proliferator-activated receptor-γ and calcitonin gene-related peptide on alcohol-induced adipogenic differentiation of bone marrow mesenchymal stem cells

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Abstract

Studies have shown that alcohol can upregulate the expression of peroxisome proliferator-activated receptor-γ (PPARγ) gene in bone marrow mesenchymal stem cells (BMSCs). High expression of PPARγ can promote adipogenic differentiation of BMSCs, and reduce their osteogenic differentiation. Abnormal proliferation of adipocytes and fatty accumulation in osteocytes can result in high intraosseous pressure and disturbance of blood circulation in the femoral head, which induces osteonecrosis of the femoral head (ONFH). Downregulation of PPARγ is efficient in inhibiting adipogenesis and maintaining osteogenesis of BMSCs, which might potentially reduce the incidence of ONFH. Calcitonin gene-related peptide (CGRP) is a neuropeptide gene which has been closely associated with bone regeneration. In this study, we aimed to observe the effect of combined regulation of the expression of PPARγ and CGRP genes on alcohol-induced adipogenic differentiation of BMSCs. Our results demonstrated that simultaneous downregulation of PPARγ and upregulation of CGRP was efficient in suppressing adipogenic differentiation of BMSCs and promoting their osteogenic differentiation. These findings might enlighten a novel approach for the prevention of ONFH.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81171776).

Conflict of interest

The authors have declared that no competing interest exists.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China

    Jinfeng Li, Yisheng Wang & Junkui Sun

  2. Basic Medical College, Zhengzhou University, Zhengzhou, 450001, Henan, China

    Yuebai Li & Guoqiang Zhao

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  1. Jinfeng Li
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  2. Yisheng Wang
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  3. Yuebai Li
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Correspondence to Yisheng Wang.

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Li, J., Wang, Y., Li, Y. et al. The effect of combined regulation of the expression of peroxisome proliferator-activated receptor-γ and calcitonin gene-related peptide on alcohol-induced adipogenic differentiation of bone marrow mesenchymal stem cells. Mol Cell Biochem 392, 39–48 (2014). https://doi.org/10.1007/s11010-014-2016-4

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  • DOI: https://doi.org/10.1007/s11010-014-2016-4

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