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Increased expression of microRNA-9 predicts an unfavorable prognosis in human glioma

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Abstract

microRNA-9 (miR-9) has been found to be upregulated along with tumor progression of gliomas by microarray-based expression profiling, and also be strongly linked to glioblastoma subtypes. However, its prognostic value in glioma is still elusive. miR-9 expression in human gliomas and nonneoplastic brain tissues was measured by real-time quantitative RT-PCR assay. miR-9 expression in glioma tissues was significantly higher than that in corresponding nonneoplastic brain tissues (P < 0.001). The increased expression of miR-9 was more frequently observed in glioma tissues with high WHO grade than those with low WHO grade tissues (P = 0.001). The expression levels of miR-9 in glioma tissues with low Karnofsky performance score (KPS) were also significantly higher than those with high KPS (P = 0.008). Moreover, the overall survival of glioma patients with high miR-9 expression was obviously lower than that with low miR-9 expression (P < 0.001). Multivariate analysis further showed that high miR-9 expression was an independent prognostic factor for overall survival in glioma patients (P = 0.01). More importantly, the subgroup analyses indicated that the overall survival of glioma patients with high WHO grade (III–IV) was significantly worse for high miR-9 expression group than for low miR-9 expression group (P < 0.001), but no significant difference was found for patients with low WHO grade (I–II). These findings suggest for the first time that the increased expression of miR-9 may play an important role in tumor progression in human gliomas. miR-9 might be a useful marker for predicting the clinical outcome of glioma patients, especially for advanced subtypes.

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Funding

This work was funded by National Natural Science Foundation of China (NO. 81272776).

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, Fourth Military Medical University, Xi’an, 710032, China

    Zhenyu Wu & Yunqing Li

  2. Department of Neurosurgery, Tangdu Hospital, Fourth military medical university, No.569, Xinsi Road, Baqiao District, Xi’an, 710038, China

    Liang Wang, Gang Li & Guodong Gao

  3. Department of Experimental Surgery, Tangdu Hospital, Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi’an, 710038, China

    Hui Liu, Feiyan Fan & Zhaobo Li

Authors
  1. Zhenyu Wu
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  2. Liang Wang
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  3. Gang Li
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  4. Hui Liu
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  5. Feiyan Fan
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  6. Zhaobo Li
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  7. Yunqing Li
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  8. Guodong Gao
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Correspondence to Yunqing Li or Guodong Gao.

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Wu, Z., Wang, L., Li, G. et al. Increased expression of microRNA-9 predicts an unfavorable prognosis in human glioma. Mol Cell Biochem 384, 263–268 (2013). https://doi.org/10.1007/s11010-013-1805-5

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  • DOI: https://doi.org/10.1007/s11010-013-1805-5

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