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Poly(I:C) induce bone marrow precursor cells into myeloid-derived suppressor cells

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Abstract

Dendritic cells (DC) and myeloid-derived suppressor cells (MDSC) are important cells involved in immune response. DC can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Recent studies have revealed that combined treatment of bone marrow MDSC with LPS plus IFN-γ inhibited the DC development but enhanced MDSC functions, such as NO release and T cell suppression. In our study, bone marrow precursor cells cultures in GM-CSF and IL-4 were treated with poly(I:C) through the culture, Gr1+CD11b+ cells with MDSC functions, such as NO release and T cell suppression were accumulated in the culture system. Then the similar phenomenon was observed in the vesicular stomatitis virus infection in vivo. In conclusion, we demonstrated that the bone marrow precursor cells in the presence of GM-CSF and IL-4 can differentiate into MDSC, which is dependent on the dynamic of interaction with poly(I:C).

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Acknowledgment

We thanks to Dr. Xuetao Cao from National Key Laboratory of Medical Immunology and Dr. Bing Yu from department of cell biology of our university for providing additional helps.

Funding

This work was supported in part by the grants from National Natural Science Foundation of China (No. 30872313 and No. 30271232).

Conflict of interest

The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai, China

    Cong Liu, Chaoxiong Zhang, Hongjuan Lu, Zhigang Wang, Jie Chen, Fang Liu & Weimin Sun

  2. Department of Radiation Medicine, Second Military Medical University, Shanghai, China

    Cong Liu & Jianming Cai

  3. Department of Centre for Disease Prevention and Control, Chengdu Military Region, Kunming, 650032, China

    Chaoxiong Zhang

  4. Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, China

    Zhumei Wu

  5. Department Gynaecology and Obstetrics, Yuxi People Hospital, Yuxi, China

    Xiaogang Liu

  6. Department of Immunology, Second Military Medical University, 800, Xiangyin Road, 200433, Shanghai, People’s Republic of China

    Weimin Sun

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  1. Cong Liu
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Correspondence to Weimin Sun.

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Liu, C., Zhang, C., Lu, H. et al. Poly(I:C) induce bone marrow precursor cells into myeloid-derived suppressor cells. Mol Cell Biochem 358, 317–323 (2011). https://doi.org/10.1007/s11010-011-0982-3

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