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The association between GSTT1, M1, and P1 polymorphisms with coronary artery disease in Western Iran

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Abstract

DNA damage which occurred by the effect of oxidant and mutant agents has an essential role in the development of atherosclerosis. To investigate the possible association between GSTs polymorphism with coronary artery disease (CAD), we investigated the frequency of GSTT1, M1, and P1 genotypes in patients with CAD compared to controls. The genotypes of GSTT1, M1, and P1 were determined in 209 angiographically documented CAD patients and 108 normal coronary artery cases (as controls) by Multiplex Polymerase Chain Reaction and PCR-RFLP. In CAD patients, the frequency of GSTT1-null genotype was significantly (P = 0.025) lower than that in control. The presence of this genotype was associated with 2.2-fold increased risk of CAD. However, the frequency of GSTM1 and GSTP1 genotypes were not significantly different comparing both groups (P = 0.405 and P = 0.521, respectively). Moreover, non smokers patients had a lower frequency of GSTM1-null genotype (29.2%) compared to non smoker controls (43.5%, P = 0.043). Also, the frequency of both GSTT1-null and GSTM1-null genotypes in patients (3.8%) was significantly lower compared to controls with the same genotypes (10.2%, P = 0.014). Our results indicated that a reduction in the frequency of GSTT1-null and GSTM1-null genotypes that observed in our study might be involved in the pathogenesis of CAD in our population.

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Acknowledgments

This study was financially supported by a grant from Kermanshah University of Medical Sciences, Kermanshah, Iran.

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Authors and Affiliations

  1. Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Hamid Nomani, Hadi Mozafari, Zohreh Rahimi & Asad Vaisi Raygani

  2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Hadi Mozafari & Zohreh Rahimi

  3. Department of Biochemistry & Genetic, School of Medicine, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

    Shahrokh Mohamadzadeh Ghobadloo

  4. Department of Internal Medicine, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mehr Ali Rahimi & Ali Asghar Keshavarz

  5. Department of Cardiology, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Asghar Fadaei Haghi

  6. Faculty Member of Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Daneshgah Avenue, Kermanshah, P.O. Box: 67155-1957, Iran

    Hadi Mozafari

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  1. Hamid Nomani
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Nomani, H., Mozafari, H., Ghobadloo, S.M. et al. The association between GSTT1, M1, and P1 polymorphisms with coronary artery disease in Western Iran. Mol Cell Biochem 354, 181–187 (2011). https://doi.org/10.1007/s11010-011-0817-2

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  • DOI: https://doi.org/10.1007/s11010-011-0817-2

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