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Renoprotective effect of Erdosteine in rats against gentamicin nephrotoxicity: a comparison of 99mTc-DMSA uptake with biochemical studies

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Abstract

Erdosteine is a mucolytic agent having antioxidant properties through its active metabolites in acute injuries induced by pharmacological drugs. This study was designed to investigate the renoprotective potential of Erdosteine against gentamicin (GM)-induced renal dysfunction by using Technetium-99 m dimercaptosuccinic acid (Tc-99 m DMSA) uptake and scintigraphy in rats. For this purpose, male Wistar rats were randomly allotted into one of the four experimental groups: Control, Erdosteine, GM, and GM + Erdosteine groups. GM and GM + Erdosteine groups received 100 mg/kg GM intramuscularly for 6 days. In addition, Erdosteine and GM + Erdosteine groups received 50 mg/kg Erdosteine orally for 6 days. Renal function tests were assessed by serum blood urea nitrogen (BUN), creatinine levels, as well as scintigraphic and tissue radioactivity measurements with Tc-99 m DMSA. Renal oxidative damage was determined by renal malondialdehyde (MDA) levels, by antioxidant enzyme activities; superoxide dismutase (SOD) and catalase (CAT) and activities of oxidant enzymes; xanthine oxidase (XO) and myeloperoxidase (MPO). GM administration resulted in marked renal lipid peroxidation, increased XO and MPO activities and decreased antioxidant enzyme activities. GM + Erdosteine group significantly had lower MDA levels, higher SOD and CAT activities and lower XO and MPO activities, when compared to GM. Also GM + Erdosteine had lower levels of serum BUN, creatinine and higher renal tissue Tc-99 m DMSA uptake and radioactivity with respect to GM. In conclusion, our results supported a protective role of Erdosteine in nephrotoxicity associated with GM treatment.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Faculty of Medicine, Zonguldak Karaelmas University, 67600 Kozlu, 44069, Zonguldak, Turkey

    Mehmet Cabuk & Feyza Sen

  2. Departments of Biochemistry, Faculty of Medicine, Zonguldak Karaelmas University, 67600 Kozlu, 44069, Zonguldak, Turkey

    Ahmet Gurel

  3. Department of Family Medicine, Faculty of Medicine, Zonguldak Karaelmas University, 67600 Kozlu, 44069, Zonguldak, Turkey

    Nejat Demircan

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  1. Mehmet Cabuk
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  2. Ahmet Gurel
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  3. Feyza Sen
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  4. Nejat Demircan
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Correspondence to Mehmet Cabuk.

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Cabuk, M., Gurel, A., Sen, F. et al. Renoprotective effect of Erdosteine in rats against gentamicin nephrotoxicity: a comparison of 99mTc-DMSA uptake with biochemical studies. Mol Cell Biochem 308, 35–42 (2008). https://doi.org/10.1007/s11010-007-9609-0

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  • DOI: https://doi.org/10.1007/s11010-007-9609-0

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