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Influence of selenium (antioxidant) on gliclazide induced hypoglycaemia/anti hyperglycaemia in normal/alloxan-induced diabetic rats

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Abstract

Oxidative stress is involved in diabetes mellitus and its complications. Since diabetes is a stress-related disorder, supplementation with antioxidants may improve the condition. The purpose of this study is to know the effect of oral administration of selenium on blood glucose and its influence on gliclazide induced hypoglycaemia/antihyperglycaemia in normal and alloxan-induced diabetic rats. Albino rats of either sex were divided into three groups of six each. Group-I/II/III were treated with selenium 1/2 TD (0.9 μg/200 g rat)/TD (1.8 μg/200 g rat)/2TD (3.6 μg/200 g rat), respectively. Later group II was treated with gliclazide TD (1.44 mg/200 g rat)/selenium TD + gliclazide TD with a washout period of 1 week between the treatments. Diabetes was induced by alloxan monohydrate 100 mg/kg body weight i.p. A group of six rats showing fasting blood glucose levels ranging from 175–250 mg/dl were selected for the study. Rats were treated with selenium TD, gliclazide TD and selenium TD + gliclazide TD with a washout period of 1 week between the treatments. Selenium 1/2 TD and TD produced hypoglycaemia while 2TD produced hyperglycaemia. The combination of selenium TD with gliclazide TD, significantly enhanced the glucose lowering effect of gliclazide in normal and diabetic rats.

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Authors and Affiliations

  1. University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, 530 003, AP, India

    S. Satyanarayana, J. Rajad Sekhar & K. Eswar Kumar

  2. Department of Biological Sciences, Alcorn State University, Lormann, MS, U.S.A.

    L. Bacchus Shannika, Bettaiya Rajanna & Sharada Rajanna

  3. Pharmacology Division, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, 530 003, Andhra Pradesh, India

    S. Satyanarayana

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  1. S. Satyanarayana
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  2. J. Rajad Sekhar
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  3. K. Eswar Kumar
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  4. L. Bacchus Shannika
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  5. Bettaiya Rajanna
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  6. Sharada Rajanna
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Correspondence to S. Satyanarayana.

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Satyanarayana, S., Sekhar, J.R., Kumar, K.E. et al. Influence of selenium (antioxidant) on gliclazide induced hypoglycaemia/anti hyperglycaemia in normal/alloxan-induced diabetic rats. Mol Cell Biochem 283, 123–127 (2006). https://doi.org/10.1007/s11010-006-2387-2

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  • DOI: https://doi.org/10.1007/s11010-006-2387-2

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