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Effects of dietary selenium on post-ischemic expression of antioxidant mRNA

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Abstract

Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion. (Mol Cell Biochem 270: 131–138, 2005)

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References

  1. Kloner R, Przyklenk K, Whittaker P: Deleterious effects of oxygen radicals in ischemia/reperfusion. Circ 80: 1115–1127, 1989

    Google Scholar 

  2. McCord J: Oxygen-derived free radicals in postischemic tissue injury. New Eng J Med 312: 159–163, 1985

    Google Scholar 

  3. Venardos K, Harrison G, Headrick J, Perkins A: Effects of dietary selenium on glutathione peroxidase and thioredoxin reductase activity and recovery from cardiac ischemia-reperfusion. J Trace Elem Med Biol 18: 81–88, 2004

    Google Scholar 

  4. Toufektsian M-C, Boucher F, Pucheu S, Tanguy S, Ribout C, Sanou D, Tresallet N, de Leiris J: Effects of selenium deficiency on the response of cardiac tissue to ischemia and reperfusion. Toxicol 148: 125–132, 2000

    Google Scholar 

  5. Polronieri R, Cevese A, Sbarbati A: Protective effect of selenium in cardiac ischemia and reperfusion. Cardioscience 3: 155–160, 1992

    Google Scholar 

  6. Weiss S, Evenson J, Thompson K, Sunde R: Dietary selenium regulation of glutathione peroxidase mRNA and other selenium-dependent parameters in male rats. J Nutr Biochem 8: 85–91, 1997

    Google Scholar 

  7. Hadley K, Sunde R: Selenium regulation of thioredoxin reductase activity and mRNA levels in rat liver. J Nutr Biochem 12: 693–702, 2001

    Google Scholar 

  8. Liu D, Liu S, Huang Y, Liu Y, Zhang Z, Han L: Effect of selenium on human myocardial glutathione peroxidase gene expression. Chin Med J (Engl) 113: 771–775, 2000

    Google Scholar 

  9. Peirson S, Butler J, Foster R: Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res 31(14): e73, 2003

    Google Scholar 

  10. Ashton K, Holmgren K, Peart J, Lankford A, Matherne G, Grimmond S, Headrick J: Effects of A1 adenosine receptor overexpression on normoxic and post-ischemic gene expression. Cardiovasc Res 57: 715–726, 2003

    Google Scholar 

  11. Dak D, Engelman R, KimuraY: Molecular adaptation of cellular defences following preconditioning of the heart by repeated ischemia. Cardiovasc Res 27: 578–584, 1993

    Google Scholar 

  12. Allan C, Lacourciere G, Stadtman T: Responsiveness of selenoproteins to dietary selenium. Annu Rev Nutr 19: 1–16, 1999

    Google Scholar 

  13. Keshan Disease Research Group: Epidemiologic studies on the etiologic relationship of selenium and Keshan disease. Chin Med 83: 546–550, 1979

    Google Scholar 

  14. Korpela H: Hepatic selenium concentrations in pigs with microangioplasty (mulberry heart disease) – an animal model for the study of oxidative damage. Int J Vitam Nutr Res 60: 156–158, 1990

    Google Scholar 

  15. Husbeck B, Berggren M, Powis G: DNA microarray reveals increased expression of thioredoxin peroxidase in thioredoxin-1 transfected cells and its functional consequences. Adv Exp Med Biol 500: 157–168, 2000

    Google Scholar 

  16. Arner E, Holmgren A: Physiological functions of thioredoxin and thioredoxin reductase. Eur J Biochem 267: 6102–6109, 2000

    Google Scholar 

  17. Gross M, Oertel M, Kohrle J: Differential selenium-dependent expression of type I 5′-deiodinase and glutathione peroxidase in the porcine epithelial cell line LLC-PK1. Biochem J 306: 851–856, 1995

    Google Scholar 

  18. Bermano G, Arthur J, Hesketh J: Selective control of cytosolic glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase mRNA stability by selenium supply. FEBS Lett 387: 157–160, 1996

    Google Scholar 

  19. Jameson R, Carlson B, Butz M, Esser K, Hatfield D, Diamond A: Selenium influences the turnover of selenocysteine tRNA[Ser]Sec chinese hamster ovary cells. J Nutr 132: 1830–1835, 1992

    Google Scholar 

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Authors and Affiliations

  1. Heart Foundation Research Centre, School of Health Science, Griffith University Gold Coast Campus, Southport, Australia

    Kylie Venardos, Kevin Ashton, John Headrick & Anthony Perkins Ph.D

  2. Heart Foundation Research Centre, School of Health Science, Griffith University Gold Coast Campus, Southport, QLD, 4217, Australia

    Anthony Perkins Ph.D

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  1. Kylie Venardos
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  2. Kevin Ashton
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  3. John Headrick
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  4. Anthony Perkins Ph.D
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Correspondence to Anthony Perkins Ph.D.

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Venardos, K., Ashton, K., Headrick, J. et al. Effects of dietary selenium on post-ischemic expression of antioxidant mRNA. Mol Cell Biochem 270, 131–138 (2005). https://doi.org/10.1007/s11010-005-5279-y

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  • DOI: https://doi.org/10.1007/s11010-005-5279-y

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