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Bone regeneration using a freeze-dried 3D gradient-structured scaffold incorporating OIC-A006-loaded PLGA microspheres based on β-TCP/PLGA

  • Tissue Engineering Constructs and Cell Substrates
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Abstract

To reveal the latent capacity of the growth factor-like low-molecular-weight material OIC-A006 in tissue regeneration, it is essential to design a porous scaffold in order to concurrently accommodate cells and drug release in a controlled manner. Consequently, we fabricated poly (l-lactide-co-glycolide) (PLGA)-based microspheres with an OIC-A006-loaded gradient-structured β-TCP/PLGA scaffold by freeze-drying which could then be used for drug delivery and bone regeneration. The OIC-A006-loaded β-TCP/PLGA scaffold consisted of two parts which loaded different doses of OIC-A006 (6.25 μM, outside; 12.5 μM, inside). The porosity, compressive strength, SEM, degradation, and cumulative amount of drug release in vitro were characterized. Furthermore, we confirmed the incorporation of OIC-A006 into the PLGA-based microspheres within the scaffolds using UV-spectrophotometry, and the amount of drug remaining in the scaffold was maintained by 10 % for up to 28 days. The drug release was slower in the normal-structured drug-loaded scaffold. The OIC-A006 released action from the OIC-A006-loaded β-TCP/PLGA scaffold with ideal the rapeutic prospects in tissue regeneration. In vitro cell culture results showed that this gradient-structured composite scaffold can induce the adhesion and proliferation of rat bone marrow stromal cells towards osteoblasts. These results showed that the newly developed OIC-A006-loaded scaffolds with gradient structure can be potentially applied to bone regeneration in clinical applications.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (NSFC) Grant funded by the Chinese government (No. 2013-81201386) and The China Postdoctoral Science Foundation (No. 20100470110).

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Authors and Affiliations

  1. School of Mechatronics Engineering and Automation, Shanghai University, Shanghai, 200444, China

    Liulan Lin, Haitao Gao & Yangyang Dong

  2. School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China

    Liulan Lin

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  1. Liulan Lin
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  2. Haitao Gao
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  3. Yangyang Dong
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Correspondence to Haitao Gao.

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Lin, L., Gao, H. & Dong, Y. Bone regeneration using a freeze-dried 3D gradient-structured scaffold incorporating OIC-A006-loaded PLGA microspheres based on β-TCP/PLGA. J Mater Sci: Mater Med 26, 3 (2015). https://doi.org/10.1007/s10856-014-5327-9

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  • DOI: https://doi.org/10.1007/s10856-014-5327-9

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