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Media-based effects on the hydrolytic degradation and crystallization of electrospun synthetic-biologic blends

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Abstract

Tissue engineering scaffold degradation in aqueous environments is a widely recognized factor determining the fate of the associated anchorage-dependent cells. Electrospun blends of synthetic polycaprolactone (PCL) and a biological polymer, gelatin, of 25, 50, and 75 wt% were investigated for alterations in crystallinity, microstructure and morphology following widely used in vitro biological exposures. To our knowledge, the effects of these different aqueous-based biological media compositions on the degradation of these blends have never been directly compared. X-ray diffraction (XRD) analysis exposed that differences in PCL crystallinity were observed following exposures to phosphate buffered solution (PBS), Dulbecco’s modified eagle medium (DMEM) cell culture media, and DI water following 7 days of exposure at 37 °C. XRD data suggested that in vitro medium exposures aid in providing chain mobility and rearrangement due to hydrolytic degradation of the gelatin phase, allowing previously constrained, poorly crystalline PCL regions to achieve more intense reflections resulting in the presence of crystalline peaks. The dry, as-spun modulus of relatively soft 100 % PCL fibers was approximately 10 % of any gelatin-containing composition. Tensile testing results indicate that hydrated gelatin containing scaffolds on average had a fivefold increase in elongation compared to as-spun scaffolds. After 24-h of aqueous exposure, the elastic modulus decreased in proportion to increasing gelatin content. After 1 day of exposure, the 75 and 100 % gelatin compositions largely ceased to display measurable values of modulus, elongation or tensile strength due to considerable hydrolytic degradation. On a relative basis, common aqueous in vitro medium exposures (deionized water, PBS, and DMEM) resulted in significantly divergent amounts of crystalline PCL, overall microstructure and fiber morphology in the blended compositions, subsequently ‘shielding’ scaffolds from significant changes in mechanical properties after 24-h of exposure. Understanding electrospun PCL-gelatin scaffold dynamics in different aqueous-based cell culture medias enables the ability to tailor scaffold composition to ‘tune’ degradation rate, microstructure, and long-term mechanical stability for optimal cellular growth, proliferation, and maturation.

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Acknowledgments

This work was supported by a research grant from the National Science Foundation under Grant No. EEC-0425626. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Ohio State University, Columbus, OH, 43210, USA

    M. Tyler Nelson

  2. Department of Materials Science and Engineering, Ohio State University, 477 Watts Hall 2041 College Rd., Columbus, OH, 43210-1179, USA

    Jed Johnson & John Lannutti

  3. Nanofiber Solutions, Columbus, OH, 43210, USA

    Jed Johnson

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  1. M. Tyler Nelson
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Correspondence to John Lannutti.

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Nelson, M.T., Johnson, J. & Lannutti, J. Media-based effects on the hydrolytic degradation and crystallization of electrospun synthetic-biologic blends. J Mater Sci: Mater Med 25, 297–309 (2014). https://doi.org/10.1007/s10856-013-5077-0

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