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Effective combination of hydrostatic pressure and aligned nanofibrous scaffolds on human bladder smooth muscle cells: implication for bladder tissue engineering

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Abstract

Bladder tissue engineering has been the focus of many studies due to its highly therapeutic potential. In this regard many aspects such as biochemical and biomechanical factors need to be studied extensively. Mechanical stimulations such as hydrostatic pressure and topology of the matrices are critical features which affect the normal functions of cells involved in bladder regeneration. In this study, hydrostatic pressure (10 cm H2O) and stretch forces were exerted on human bladder smooth muscle cells (hBSMCs) seeded on aligned nanofibrous polycaprolactone/PLLA scaffolds, and the alterations in gene and protein expressions were studied. The gene transcription patterns for collagen type I, III, IV, elastin, α-SMA, calponin and caldesmon were monitored on days 3 and 5 quantitatively. Changes in the expressions of α-SMA, desmin, collagen type I and III were quantified by Enzyme-linked immuno-sorbent assay. The scaffolds were characterized using scanning electron microscope, contact angle measurement and tensile testing. The positive effect of mechanical forces on the functional improvement of the engineered tissue was supported by translational down-regulation of α-SMA and VWF, up-regulation of desmin and improvement of collagen type III:I ratio. Altogether, our study reveals that proper hydrostatic pressure in combination with appropriate surface stimulation on hBSMCs causes a tissue-specific phenotype that needs to be considered in bladder tissue engineering.

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Acknowledgments

This work was supported financially by University of Tehran and Stem Cell Technology Research Center. The authors wish to thank Dr. E. C. Thrower, Yale University, for critical revision of the manuscript.

Conflict of interest

Authors have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Membrane Biophysics and Macromolecules, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Hana Hanaee Ahvaz & Hamid Mobasheri

  2. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Hana Hanaee Ahvaz, Masoud Soleimani, Behnaz Bakhshandeh, Sara Soudi & Maryam Hafizi

  3. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran

    Masoud Soleimani

  4. Biomaterials Research Center (BRC), University of Tehran, Tehran, Iran

    Hamid Mobasheri

  5. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

    Behnaz Bakhshandeh

  6. Urology and Nephrology Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran

    Naser Shakhssalim

  7. Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Vasei

  8. Nanotechnology and Tissue Engineering Department, Stem Cell Technology Research Center, Tehran, Iran

    Masumeh Dodel

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  1. Hana Hanaee Ahvaz
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  2. Masoud Soleimani
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Correspondence to Masoud Soleimani or Hamid Mobasheri.

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Dedicated to late Professor M. N. Sarbolouki.

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Ahvaz, H.H., Soleimani, M., Mobasheri, H. et al. Effective combination of hydrostatic pressure and aligned nanofibrous scaffolds on human bladder smooth muscle cells: implication for bladder tissue engineering. J Mater Sci: Mater Med 23, 2281–2290 (2012). https://doi.org/10.1007/s10856-012-4688-1

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  • DOI: https://doi.org/10.1007/s10856-012-4688-1

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