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Nanomechanics and Raman spectroscopy of fibrillin 2 knock-out mouse bones

  • Nano- and micromechanical properties of hierarchical biological materials
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Abstract

Absence of fibrillin 2 (Fbn2), a non-collagenous bone protein, causes a connective tissue disorder called congenital contractural arachnodactyly (CCA) and has been associated with decreased bone mineral density. Nanoindentation and Raman microspectroscopy have been used to compare the mechanical and chemical properties of cortical bone from femora of Fbn2−/− deficient mice and their wild-type controls (Fbn2+/+). It was found that Fbn2−/− bones have significantly lower hardness and elastic modulus compared to Fbn2+/+ bones, especially in the mid-cortical section. The Raman analysis showed little difference with genotype except for a decrease in type-B carbonate substitution in the endosteal region of Fbn2−/− bones. The results indicate that Fbn2 plays a direct role in determining the mechanical properties of bone.

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Acknowledgments

The authors would like to thank Prof. David Denhardt of Rutgers University, NJ and Prof. Nejat Guzelsu of UMDNJ, NJ for helpful discussions. Financial support for this research has been provided by NSF, ACS PRF, DOD DURIP, NJCHE, NIH and the Rutgers University Busch Bequest.

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Authors and Affiliations

  1. Materials Science & Engineering, Rutgers University, Piscataway, NJ, 08854, USA

    N. B. Kavukcuoglu & A. B. Mann

  2. Child Health Institute, UMDNJ-RWJMS, New Brunswick, NJ, 08903, USA

    E. Arteaga-Solis, S. Lee-Arteaga & F. Ramirez

  3. Biomedical Engineering, Rutgers University, Piscataway, NJ, 08854, USA

    A. B. Mann

Authors
  1. N. B. Kavukcuoglu
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  2. E. Arteaga-Solis
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  3. S. Lee-Arteaga
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  4. F. Ramirez
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  5. A. B. Mann
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Correspondence to A. B. Mann.

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Kavukcuoglu, N.B., Arteaga-Solis, E., Lee-Arteaga, S. et al. Nanomechanics and Raman spectroscopy of fibrillin 2 knock-out mouse bones. J Mater Sci 42, 8788–8794 (2007). https://doi.org/10.1007/s10853-007-1918-x

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  • DOI: https://doi.org/10.1007/s10853-007-1918-x

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