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Calcifying epithelial odontogenic tumor: an immunohistochemical case study

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Abstract

Calcifying epithelial odontogenic tumor (CEOT) is a rare benign odontogenic tumor. A case of CEOT in a 25-year-old female is presented here. Histologically, the case showed sheets of polyhedral epithelial cells with deep eosinophilic cytoplasm and prominent nuclei. Nuclear pleomorphism and hyperchromatism were evident. Globules of amyloid-like material among the tumor cells were prominent. Also found was a small area demonstrating a cribriform pattern. Immunohistochemical studies with antibodies against basement membrane proteins (laminins 1 and 5, collagen type IV and fibronectin), pan-cytokeratins AE1/AE3, vimentin, S-100 protein and CD 1a were performed. Tumor cells expressed laminins 1 and 5, fibronectin, cytokeratins and vimentin. The amyloid-like material was not reactive to all antibodies examined. A number of dendritic cells among sheets of tumor cells were revealed with strong staining for S-100 protein and CD 1a. These dendritic cells are likely to be Langerhans cells. Hence, immunohistochemistry is a useful method to study the variant of CEOT.

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Acknowledgments

The authors would like to thank Dr. Thanapun Leelanuntakij (Pranungkao Hospital) for his contribution of surgical specimens, Professor Chainut Chongruk for valuable comment, Mrs. Unruen Meesakul for her technical assistance and Dr Justin Otzen Meiland for reviewing the manuscript. We are indebted to Dr. Paninee Thavarungkul (Institute of Pathology, Ministry of Public Health) for interpreting the Congo red-stained slide.

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Authors and Affiliations

  1. Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Yothi Street, Bangkok, 10400, Thailand

    Sopee Poomsawat & Jirapa Punyasingh

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  1. Sopee Poomsawat
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  2. Jirapa Punyasingh
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Correspondence to Sopee Poomsawat.

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Poomsawat, S., Punyasingh, J. Calcifying epithelial odontogenic tumor: an immunohistochemical case study. J Mol Hist 38, 103–109 (2007). https://doi.org/10.1007/s10735-007-9082-9

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  • DOI: https://doi.org/10.1007/s10735-007-9082-9

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