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Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque)

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Abstract

Protein arginine methylation, catalyzed by protein arginine methyltransferases (PRMT), has recently emerged as an important modification in the regulation of gene expression. In this communication, we identified and characterized the channel catfish orthologs to human PRMT 1, 3, 4 and 5, and PRMT4 like. Each PRMT nucleic acid sequence has an open reading frame (ORF) and 3′-untranslated regions. Each ORF appears to encode 361, 587 and 458 amino acid residues for PRMT1, PRMT4 and variant, respectively. The partial ORF of PRMT3 and PRMT5 encode 292 and 563 amino acids, respectively. By comparison with the human counterparts, each channel catfish PRMT also has conserved domains. For expression profile, the channel catfish PRMT1 transcript was detected by RT-PCR in spleens, anterior kidneys, livers, intestines, skin and gills of fish examined. Except in liver, the PRMT3 transcript was detected in all catfish tissues examined. However, the PRMT4 cDNA was detected in livers from all three catfish and gills from two fish, but not other tissues. This information will enable us to further elucidate PRMT functions in channel catfish.

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Acknowledgments

We are grateful to Mr. Jason White of the USDA ARS Aquatic Animal Health Research Unit, Auburn, AL, for excellent technical support, and Dr. Brian E. Scheffler and his Bioinformatics Group at the USDA ARS Genomics and Bioinformatics Research Unit in Stoneville, MS, for DNA sequencing and bioinformatics. We also thank Dr. Brian B. Oakley of Poultry Microbiological Safety Research Unit, ARS, USDA, Athens, GA, for construction of the phylogenetic tree. This study was supported by the USDA Agricultural Research Service CRIS project no. 6420-32000-024-00D. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer.

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  1. United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL, 36832-4352, USA

    Hung-Yueh Yeh & Phillip H. Klesius

  2. Poultry Microbiological Safety Research Unit, United States Department of Agriculture, Richard B. Russell Agricultural Research Center, Agricultural Research Service, 950 College Station Road, Athens, GA, 30605-2720, USA

    Hung-Yueh Yeh

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Yeh, HY., Klesius, P.H. Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque). Fish Physiol Biochem 38, 1083–1098 (2012). https://doi.org/10.1007/s10695-011-9593-x

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