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Protective Effects of Arctium lappa L. Roots Against Hydrogen Peroxide-Induced Cell Injury and Potential Mechanisms in SH-SY5Y Cells

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Abstract

Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of Arctium lappa L. roots (EAL) on hydrogen peroxide (H2O2)-induced cell injury in human SH-SY5Y neuroblastoma cells. The cell viability was significantly decreased after exposure to 200 μM H2O2, whereas pretreatment with different concentrations of EAL attenuated the H2O2-induced cytotoxicity. Hoechst 33342 staining indicated that EAL reversed nuclear condensation in H2O2-treated cells. Meanwhile, TUNEL assay with DAPI staining showed that EAL attenuated apoptosis was induced by H2O2. Pretreatment with EAL also markedly elevated activities of antioxidant enzyme (GSH-Px and SOD), reduced lipid peroxidation (MDA) production, prevented ROS formation, and the decrease of mitochondrial membrane potential. In addition, EAL showed strong radical scavenging ability in 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays. Furthermore, EAL inhibited H2O2-induced apoptosis by increases in the Bcl-2/Bax ratio, decreases in cytochrome c release, and attenuation of caspase-3, caspase-9 activities, and expressions. These findings suggest that EAL may be regarded as a potential antioxidant agent and possess potent neuroprotective activity against H2O2-induced injury.

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Acknowledgments

This project was supported by the Key National Science & Technology Specific Project of China (2014ZX09J14101-05C).

Conflict of interest

Xing Tian, Li-Ping Guo, Xiao-Long Hu, Jin Huang, Yan-Hua Fan, Tian-Shu Ren and Qing-Chun Zhao declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China

    Xing Tian, Li-Ping Guo, Xiao-Long Hu, Jin Huang, Yan-Hua Fan, Tian-Shu Ren & Qing-Chun Zhao

  2. Department of Life Science and Biochemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Xing Tian & Qing-Chun Zhao

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  1. Xing Tian
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  2. Li-Ping Guo
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Correspondence to Qing-Chun Zhao.

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Tian, X., Guo, LP., Hu, XL. et al. Protective Effects of Arctium lappa L. Roots Against Hydrogen Peroxide-Induced Cell Injury and Potential Mechanisms in SH-SY5Y Cells. Cell Mol Neurobiol 35, 335–344 (2015). https://doi.org/10.1007/s10571-014-0129-7

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