Abstract
(1) Phase II enzyme inducer is a kind of compound which can promote the expression of antioxidative enzymes through nuclear factor erythroid 2-related factor 2 (Nrf2) activation. Recently, it has been reported that these compounds show neuroprotective effect via combating oxidative stress. The purpose of this study is to determine whether phase II enzyme inducers have neuroprotective effects on traumatic spinal cord injury. (2) An organotypic spinal cord culture system was used, Phase II enzyme inducers were added to culture medium for 1 week, motor neurons were counted by SMI-32 staining, glutamate, Nrf2, and Heme oxygenase-1(HO-1) mRNA were tested. (3) This study showed motor neuron loss within 1 week in culture. After 1 week in culture, the system was stable. Moreover, Glutamate was increased when in culture 48 h and decreased after 1 week in culture. There was no significant change between 1 and 4 weeks in culture. Necrotic motor neuron and damaged mitochondrial were observed in culture 48 h. Furthermore, phase II enzyme inducers: tert-butyhydroquinone (t-BHQ), 3H-1,2-dithiole-3-thione (D3T), and 5,6-dihydrocyclopenta-1,2-dithiole-3-thione (CPDT) were shown to promote motor neuron survival after dissection, it was due to increasing Nrf2 and HO-1 mRNA expression and protecting mitochondrial not due to decreasing glutamate level. (4) The loss of motor neuron due to dissection can mimic severe traumatic spinal cord injury. These results demonstrate that glutamate excitotoxicity and the damage of mitochondrial is possibly involve in motor neuron death after traumatic spinal cord injury and phase II enzyme inducers show neuroprotective potential on motor neuron survival in traumatic spinal cord injury in vitro.
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Acknowledgment
This study was supported by grant from the Natural Science Foundation of China (No.30670732). And we thank Dr. Munday (Ruakura Agricultural Research Center, Hamilton, New Zealand) and Dr. YueSheng Zhang (Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, USA) who direct the work and provide the reagents.
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Liu, XY., Li, CY., Bu, H. et al. The Neuroprotective Potential of Phase II Enzyme Inducer on Motor Neuron Survival in Traumatic Spinal Cord Injury In vitro. Cell Mol Neurobiol 28, 769–779 (2008). https://doi.org/10.1007/s10571-007-9219-0
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DOI: https://doi.org/10.1007/s10571-007-9219-0