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Transdentinal diffusion and cytotoxicity of self-etching adhesive systems

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Abstract

Objective

To evaluated the transdentinal diffusion and subsequent cytotoxicity of self-etching adhesives on odontoblast-like cells.

Materials and methods

Sixty dentin disks (0.4-mm thick) were produced from human molars and divided into six groups (n = 10). The dentin disks were placed in in vitro pulp chambers where MDPC-23 cells were planted on 0.28 cm2 of exposed dentin on the pulpal side. The adhesives Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Adper Prompt (PR), and Xeno III (XE) were applied on the occlusal side. Single Bond (SB) was used as positive and phosphate buffer solution (PBS) as negative control. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM. The transdentinal diffusion was qualified by GC/MS.

Results

Kruskal–Wallis and Mann–Whitney tests demonstrated a significant difference among the adhesives and PBS. Cellular viability reduction promoted by the self-etching systems was lower than that of SB (53.1%), except for CSE. Cell metabolism was reduced in 47.8%, 42.1%, 28.0%, and 46.5% for CSE, CPB, PR, and XE, respectively. HEMA was identified as the main diffused component.

Conclusion

Components from all investigated self-etching adhesive systems were able to diffuse through the dentin resulting in significant reduction of the cellular metabolism.

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Acknowledgment

This study was financially supported by the Brazilian research agencies CNPq (grant 475134/2004-4) and FAPESP (grant 2006/53906-3). The authors are grateful to Dr. Elliot Kitajima and his graduate students from the NAP/MEPA–ESALQ/USP for SEM technical support.

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Authors and Affiliations

  1. Department of Oral Clinic, Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Department of Pediatric Dentistry, School of Dentistry, PUC-Minas, Belo Horizonte, MG, Brazil

    Célia Regina Moreira Lanza

  2. Department of Physiology and Pathology, Araraquara School of Dentistry, São Paulo State University—UNESP, Araraquara, SP, Brazil

    Carlos Alberto de Souza Costa

  3. Department of Organic Chemistry, Araraquara Institute of Chemistry, São Paulo State University—UNESP, Araraquara, SP, Brazil

    Maysa Furlan & Alberto Alécio

  4. Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University—UNESP, Rua Humaitá, 1680 Sala 118, 1° andar, Araraquara, SP, 14801-903, Brazil

    Josimeri Hebling

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  1. Célia Regina Moreira Lanza
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  2. Carlos Alberto de Souza Costa
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  5. Josimeri Hebling
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Correspondence to Josimeri Hebling.

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Lanza, C.R.M., de Souza Costa, C.A., Furlan, M. et al. Transdentinal diffusion and cytotoxicity of self-etching adhesive systems. Cell Biol Toxicol 25, 533–543 (2009). https://doi.org/10.1007/s10565-008-9110-x

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  • DOI: https://doi.org/10.1007/s10565-008-9110-x

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