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The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C

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Abstract

Diazinon is one of the most widely used organophosphate insecticides (OPIs) in agriculture and public health programs. Reactive oxygen species (ROS) caused by OPIs may be involved in the toxicity of various pesticides. The aim of this study was to investigate how diazinon affects lipid peroxidation (LPO) and the antioxidant defense system in vivo and the possible ameliorating role of vitamins E and C. For this purpose, experiments were done to study the effects of DI on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in adult rat heart. Experimental groups were: (1) control group, (2) diazinon treated (DI) group, (3) DI+vitamins E and C-treated (DI+Vit) group. The levels of malondialdehyde (MDA) and the activities of SOD and CAT increased significantly in the DI group compared with the control group. The activity of SOD and the levels of MDA decreased significantly in the DI+Vit group compared with the DI group. The differences between the DI+Vit and control groups according to the MDA levels and the activities of both SOD and CAT were statistically significant. These results suggest that treating rats with a single dose of diazinon increases LPO and some antioxidant enzyme activities in the rat myocardium and, in addition, that single-dose treatment with a combination of vitamins E and C after the administration of diazinon can reduce LPO caused by diazinon, though this treatment was not sufficiently effective to reduce the values to those in control group.

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Abbreviations

CAT:

catalase

DI:

diazinon

GSH-Px:

glutathione peroxidase

LPO:

lipid peroxidation

MDA:

malondialdehyde

OPI:

organophosphate insecticides

ROS:

reactive oxygen species

SOD:

superoxide dismutase (SOD)

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Authors and Affiliations

  1. Department of Biochemistry and Clinical Biochemistry, Suleyman Demirel University, School of Medicine, 32260, Isparta, Turkey

    O. Akturk, H. Demirin, R. Sutcu, N. Yilmaz & I. Altuntas

  2. Department of Physiology, School of Medicine, Suleyman Demirel University, Isparta, Turkey

    H. Koylu

Authors
  1. O. Akturk
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  2. H. Demirin
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  3. R. Sutcu
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  4. N. Yilmaz
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  5. H. Koylu
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  6. I. Altuntas
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Correspondence to O. Akturk.

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Akturk, O., Demirin, H., Sutcu, R. et al. The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C. Cell Biol Toxicol 22, 455–461 (2006). https://doi.org/10.1007/s10565-006-0138-5

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  • DOI: https://doi.org/10.1007/s10565-006-0138-5

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