Abstract
Objectives
To demonstrate that miR-9 inhibits autophagy by down-regulating Beclin1 and thus enhances the sensitivity of A549 cells to cisplatin.
Results
MiR-9 inhibited Beclin1 expression by binding to its 3′UTR. The inhibition decreased the cisplatin-induced autophagy in A549 cells, evidenced by the decreased expression of LC3II and GFP-LC3 puncta and the increased expression of P62. Upregulation of miR-9 level enhanced the sensibility of A549 cells to cisplatin and increased the cisplatin-induced apoptosis. Overexpression of Beclin1 reversed above effects of miR-9 mimics, cisplatin-induced autophagy was increased and apoptosis was decreased.
Conclusions
MiR-9 inhibits autophagy via targeting Beclin1 3′UTR and thus enhances cisplatin sensitivity in A549 cells.
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Acknowledgements
This study was funded by the National Major Special Program of New Drug Research and Development (No. 2013ZX09301303-005), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions and Qing Lan Project, National Natural Science Foundation of China, No. 81372331, and the Fundamental Research Funds for the Central Universities, No. 2015ZD004.
Supporting information
Supplementary Table 1—Primers of Beclin1 and GAPDH.
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Zhang, Y., Meng, X., Li, C. et al. MiR-9 enhances the sensitivity of A549 cells to cisplatin by inhibiting autophagy. Biotechnol Lett 39, 959–966 (2017). https://doi.org/10.1007/s10529-017-2325-2
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DOI: https://doi.org/10.1007/s10529-017-2325-2