Abstract
The bacterial superantigen staphylococcal enterotoxin B (SEB) is a potent inducer of cytotoxic T-cell activity and cytokine production in vivo. We investigated the possibility of the therapeutic application of SEB in patients with fibrosarcoma. The anti-tumor effect of SEB in mice with inoculated fibrosarcoma (WEHI-164) was examined by intravenous (IV) and intratumoral (IT) injection and the sizes of the inoculated tumors, IFN-γ production, and CD4+/CD8+ T cell infiltration were determined. The inoculated tumors were also examined histologically. In the mice in the IV-injected group, a significant reduction (P < 0.02) of tumor size was observed in comparison with mice in the IT-injected and control groups. Furthermore, the mice in the IV-injected group showed significantly higher levels of IFN-γ (P < 0.009) and CD4+/CD8+ T cell infiltration when compared with the other groups (P < 0.02). A significantly higher frequency of necrosis in tumor tissues was also observed in mice in the IV-injected group (P < 0.05). Our present findings suggest that tumor cell death is caused by increased cytotoxic T-cell activity and cytokine levels in response to the IV injection of SEB and that SEB may be a good option for use as a novel therapy in patients with fibrosarcoma.
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Acknowledgments
We are grateful to Emiko Kondo (Tohoku University School of Medicine) for her excellent technical assistance in the TUNEL assay, and to Dr. Barbara Lee Smith Pierce (University of Maryland University College) for editorial work in the preparation of this manuscript. This work was supported in part by grants from the Tarbiat Modarres and Baqiyatallah Universities, Iran, and Grants-in-Aid, the 21st Century COE Program Special Research Grant, and Academic Frontier Project for Private Universities: matching fund subsidy 2006–2010 from the Ministries of Education, Culture, Sports, Science and Technology of Japan, and by a Grant-in-Aid for Cancer Research from the Ministry of Health, Labour and Welfare of Japan.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-008-9820-4
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Fooladi, A.A.I., Sattari, M., Hassan, Z.M. et al. In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin. Biotechnol Lett 30, 2053–2059 (2008). https://doi.org/10.1007/s10529-008-9805-3
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DOI: https://doi.org/10.1007/s10529-008-9805-3