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Variations in Tendon Stiffness Due to Diets with Different Glycotoxins Affect Mechanical Properties in the Muscle-Tendon Unit

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Abstract

Passive elastic behavior of tendon tissue from rats subjected to different dietary treatments was characterized. For that purpose, twenty-four weanling Wistar rats (41.02  ±  0.16 g) were randomly distributed into four groups. During 88 days each group was fed on different diets: control diet and diets containing advanced glycation end products (AGEs) from glucose–lysine model system, from bread crust and bread dough, respectively. After the trial animals were sacrificed and tendon samples were extracted and tested mechanically to fracture in a uniaxial tensile test machine. A transversely-hyperelastic model was formulated based on stress–strain relationships and its parameters were fit to the experimental data using the Levenberg–Marquardt optimization algorithm. Material parameters were incorporated in a finite element model to study different stress–strain distributions in a muscle-tendon unit. Results show higher strains and stresses in the muscle belly when properties of a stiffer tendon associated with a diet rich in AGEs are included in the model. A real increase in this mechanical response of the tissue could imply possible pain in joint mobility.

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Acknowledgments

The authors gratefully acknowledge research support from the Spanish Ministry of Science and Technology through the research project DPI2011-27939-C02-01 as well as the financial support of the Junta de Andalucia. We also thank the Tissue Characterization Platform of CIBER-BBN for their technical support during development of the experimental tests.

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Authors and Affiliations

  1. Aragón Institute of Engineering Research, University of Zaragoza, Zaragoza, Spain

    J. Grasa & B. Calvo

  2. Centro de Investigación Biomedica en Red en Bioingeniera, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza, Spain

    J. Grasa & B. Calvo

  3. Unidad de Nutrición Animal, Estación Experimental del Zaidin, CSIC, Granada, Spain

    C. Delgado-Andrade & M. P. Navarro

Authors
  1. J. Grasa
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  2. B. Calvo
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  3. C. Delgado-Andrade
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  4. M. P. Navarro
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Correspondence to J. Grasa.

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Associate Editor Eiji Tanaka oversaw the review of this article.

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Grasa, J., Calvo, B., Delgado-Andrade, C. et al. Variations in Tendon Stiffness Due to Diets with Different Glycotoxins Affect Mechanical Properties in the Muscle-Tendon Unit. Ann Biomed Eng 41, 488–496 (2013). https://doi.org/10.1007/s10439-012-0674-5

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  • DOI: https://doi.org/10.1007/s10439-012-0674-5

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