Abstract
Melon necrotic spot virus (MNSV) is transmitted by the fungus Olpidiumbornovanus. In this study, we used immunofluorescence microscopy to detect MNSV particles over the entire surface of the O. bornovanus zoospore; MNSV particles were not detected on the related fungus O. virulentus, which cannot transmit MNSV. The amino acid substitution Ile → Phe at position 300 in the MNSV coat protein resulted in loss of both specific binding and fungal transmission, while virion assembly and biological aspects were unaffected. Taken together, these results suggest that the MNSV coat protein acts as a ligand to the O. bornovanus zoospore as part of a fungal-vector transmission system.
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This study was supported in part by a grant-in-aid of the research project for utilizing advanced technologies in agriculture, forestry and fisheries administered by the ministry of agriculture, forestry and fisheries in Japan.
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Mochizuki, T., Ohnishi, J., Ohki, T. et al. Amino acid substitution in the coat protein of Melon necrotic spot virus causes loss of binding to the surface of Olpidium bornovanus zoospores. J Gen Plant Pathol 74, 176–181 (2008). https://doi.org/10.1007/s10327-008-0080-x
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DOI: https://doi.org/10.1007/s10327-008-0080-x