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DNA repair gene expression in biological tissues exposed to low-intensity infrared laser

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Abstract

Special properties of laser light have led to its usefulness in many applications in therapy. Excitation of endogenous chromophores in biotissues and generation of free radicals could be involved in its biological effects. DNA lesions induced by free radicals are repaired by base excision repair pathway. In this work, we evaluated the expression of APE1 and OGG1 genes related to repair of DNA lesions induced by free radicals. Skin and muscle tissues of Wistar rats were exposed to low-intensity infrared laser at different fluences and frequencies. After laser exposition of 1 and 24 h, tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of APE1 and OGG1 gene expression by quantitative polymerase chain reaction. Data obtained show that laser radiation alters the expression of APE1 and OGG1 mRNA differently in skin and muscle tissues of Wistar rats depending of the fluence, frequency, and time after exposure. Our study suggests that low-intensity infrared laser affects expression of genes involved in repair of DNA lesions by base excision repair pathway.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).

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Authors and Affiliations

  1. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, 20550900, Brazil

    Adenilson de Souza da Fonseca, Andre Luiz Mencalha, Vera Maria Araújo de Campos, Samara Cristina Ferreira Machado & Antonio Augusto de Freitas Peregrino

  2. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil

    Adenilson de Souza da Fonseca

  3. Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense, Rua Outeiro de São João Batista, s/n, Niterói, 24020-140, Brazil

    Samara Cristina Ferreira Machado

  4. Setor de Facomatoses do Serviço de Genética Clínica do IPPMG, Universidade Federal do Rio de Janeiro, Avenida Brigadeiro Trompowsky, Rio de Janeiro, 21949590, Brazil

    Mauro Geller

  5. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil

    Flavia de Paoli

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  1. Adenilson de Souza da Fonseca
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  2. Andre Luiz Mencalha
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  3. Vera Maria Araújo de Campos
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  7. Flavia de Paoli
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Correspondence to Adenilson de Souza da Fonseca.

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de Souza da Fonseca, A., Mencalha, A.L., Araújo de Campos, V.M. et al. DNA repair gene expression in biological tissues exposed to low-intensity infrared laser. Lasers Med Sci 28, 1077–1084 (2013). https://doi.org/10.1007/s10103-012-1191-3

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  • DOI: https://doi.org/10.1007/s10103-012-1191-3

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