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Neurotrophic activity of human adipose stem cells isolated from deep and superficial layers of abdominal fat

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Abstract

New approaches to the clinical treatment of traumatic nerve injuries may one day utilize stem cells to enhance nerve regeneration. Adipose-derived stem cells (ASC) are found in abundant quantities and can be harvested by minimally invasive procedures that should facilitate their use in such regenerative applications. We have analyzed the properties of human ASC isolated from the deep and superficial layers of abdominal fat tissue obtained during abdominoplasty procedures. Cells from the superficial layer proliferate significantly faster than those from the deep layer. In both the deep and superficial layers, ASC express the pluripotent stem cell markers oct4 and nanog and also the stro-1 cell surface antigen. Superficial layer ASC induce the significantly enhanced outgrowth of neurite-like processes from neuronal cell lines when compared with that of deep layer cells. However, analysis by reverse transcription with the polymerase chain reaction and by enzyme-linked immunosorbent assay has revealed that ASC isolated from both layers express similar levels of the following neurotrophic factors: nerve growth factor, brain-derived neurotrophic factor and glial-derived neurotrophic factor. Thus, human ASC show promising potential for the treatment of traumatic nerve injuries. In particular, superficial layer ASC warrant further analysis of their neurotrophic molecules.

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Authors and Affiliations

  1. Department of Integrative Medical Biology, Section of Anatomy, Umeå University, SE-901 87, Umeå, Sweden

    Daniel F. Kalbermatten, Dominique Schaakxs, Paul J. Kingham & Mikael Wiberg

  2. Department of Surgical & Perioperative Science, Section of Hand and Plastic Surgery, Umeå University, Umeå, Sweden

    Mikael Wiberg

  3. Division of Plastic, Reconstructive & Aesthetic Surgery, CHUV, University Hospital of Lausanne, Lausanne, Switzerland

    Daniel F. Kalbermatten & Dominique Schaakxs

  4. Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Basel, Basel, Switzerland

    Daniel F. Kalbermatten

Authors
  1. Daniel F. Kalbermatten
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  2. Dominique Schaakxs
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  3. Paul J. Kingham
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  4. Mikael Wiberg
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Corresponding author

Correspondence to Paul J. Kingham.

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Grant support

This study was supported by the Swedish Medical Research Council, Umeå University, County of Västerbotten, Magn. Bergvalls Stiftelse, Clas Groschinskys Minnesfond, Åke Wibergs Stiftelse, the Gunvor and Josef Aner Foundation, SwissLife Foundation, the SUVA and the University of Lausanne FBM.

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Kalbermatten, D.F., Schaakxs, D., Kingham, P.J. et al. Neurotrophic activity of human adipose stem cells isolated from deep and superficial layers of abdominal fat. Cell Tissue Res 344, 251–260 (2011). https://doi.org/10.1007/s00441-011-1142-5

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  • DOI: https://doi.org/10.1007/s00441-011-1142-5

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