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5-HT1A receptor expression in pyramidal neurons of cortical and limbic brain regions

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Abstract

We studied expression of the 5-HT1A receptor in cortical and limbic areas of the brain of the tree shrew. In situ hybridization with a receptor-specific probe and immunocytochemistry with various antibodies was used to identify distinct neurons expressing the receptor. In vitro receptor autoradiography with 3H-8-OH-DPAT (3H-8-hydroxy-2-[di-n-propylamino]tetralin) was performed to visualize receptor-binding sites. In the prefrontal, insular, and occipital cortex, 5-HT1A receptor mRNA was expressed in pyramidal neurons of layer 2, whereas 3H-8-OH-DPAT labeled layers 1 and 2 generating a columnar-like pattern in the prefrontal and occipital cortex. In the striate and ventral occipital cortex, receptor mRNA was present within layers 5 and 6 in pyramidal neurons and Meynert cells. Pyramid-like neurons in the claustrum and anterior olfactory nucleus also expressed the receptor. Principal neurons in hippocampal region CA1 expressed 5-HT1A receptor mRNA, and 3H-8-OH-DPAT labeled both the stratum oriens and stratum radiatum. CA3 pyramidal neurons displayed low 5-HT1A receptor expression, whereas granule neurons in the dentate gyrus revealed moderate expression of this receptor. In the amygdala, large pyramid-like neurons in the basal magnocellular nucleus strongly expressed the receptor. Immunocytochemistry with antibodies against parvalbumin, calbindin, and gamma aminobutyric acid (GABA) provided no evidence for 5-HT1A receptor expression in GABAergic neurons in cortical and limbic brain areas. Our data agree with previous findings showing that the 5-HT1A receptor mediates the modulation of glutamatergic neurons. Expression in the limbic and cortical areas suggested an involvement of 5-HT1A receptors in emotional and cognitive processes.

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Acknowledgements

The technical assistance of Stefanie Gleisberg and Miriam Vorwald is gratefully acknowledged. We also thank Nashat Abumaria for help with the 5-HT1A receptor clone.

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  1. Clinical Neurobiology Laboratory, German Primate Center, Kellerweg 4, 37077, Göttingen, Germany

    Monika Palchaudhuri & Gabriele Flügge

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  1. Monika Palchaudhuri
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Correspondence to Gabriele Flügge.

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This work was supported by the German Science Foundation (SFB 406; C4 to G.F.).

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Palchaudhuri, M., Flügge, G. 5-HT1A receptor expression in pyramidal neurons of cortical and limbic brain regions. Cell Tissue Res 321, 159–172 (2005). https://doi.org/10.1007/s00441-005-1112-x

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