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Expression of growth hormone and its receptor in the lungs of embryonic chicks

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Abstract

The lung is well established as being a postnatal target site for growth hormone (GH) action, since pathophysiological states of GH excess and deficiency are both associated with impaired pulmonary function. Pituitary GH is therefore probably involved in normal lung growth or development, although perinatal lung development occurs prior to the differentiation of pituitary somatotrophs and the ontogeny of pituitary GH secretion. The lung itself may, however, be a site of GH production during prenatal development, since a specific GH-response gene (a marker of GH activity) is expressed in the lungs of early chick embryos, in which GH immunoreactivity is widespread in many other peripheral tissues. We have assessed this possibility in embryonic chicks. A 690-bp cDNA, identical in size and nucleotide sequence to the full-length pituitary GH transcript, was amplified by reverse transcription/polymerase chain reaction from total RNA extracted from the lungs of embryos at 11, 13, 15, and 18 days of the 21-day incubation period. This transcript was localized by in situ hybridization to mesenchymal and epithelial cells of the developing lungs, in which specific GH immunoreactivity was similarly located. Intense GH immunoreactivity was also present after embryonic day 15 (ED15) in the smooth muscle surrounding blood vessels in the lung and surrounding the bronchioles. Lung GH immunoreactivity was primarily associated with a 15-kDa protein, rather than the 26-kDa protein in the pituitary gland. After the onset of pituitary GH secretion (at ED17), GH mRNA was barely detectable in the lungs of ED20 embryos, at the start of lung breathing. GH immunoreactivity was, however, still present in some cells in the lungs of ED20 embryos. GH-receptor mRNA and immunoreactivity were also widespread and abundant within the embryonic lung. Lung GH may thus have autocrine or paracrine roles in lung development or in pulmonary function prior to the ontogeny of the pituitary gland and the appearance of GH in peripheral plasma.

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Authors and Affiliations

  1. Department of Physiology and Perinatal Research Centre, University of Alberta, Edmonton, T6G 2H7, Canada

    Jason A. Beyea, Richard A. Vandergriend & Steve Harvey

  2. Departments of Obstetrics & Gynecology and Pediatrics, University of Alberta, Edmonton, T6G 2H7, Canada

    David M. Olson

  3. Department of Physiology, 7-41 Medical Sciences Building, Edmonton, Alberta, T6G 2H7, Canada

    Steve Harvey

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  1. Jason A. Beyea
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  2. David M. Olson
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  3. Richard A. Vandergriend
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  4. Steve Harvey
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Correspondence to Steve Harvey.

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The authors thank the Natural Sciences & Engineering Research Council of Canada (NSERC; S.H.), the Canadian Institute for Health Research, and the Hospital for Sick Children Foundation (D.M.O.) for financial support. J.B. was in receipt of studentships from the Alberta Foundation for Medical Research, from NSERC, and from a CIHR Strategic Training Grant in Maternal, Fetal and Newborn Health.

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Beyea, J.A., Olson, D.M., Vandergriend, R.A. et al. Expression of growth hormone and its receptor in the lungs of embryonic chicks. Cell Tissue Res 322, 379–392 (2005). https://doi.org/10.1007/s00441-005-0040-0

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