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Cryptosporidium parvum oocyst viability and behaviour of the residual body during the excystation process

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Abstract

This study was conducted as a comparative evaluation of time-dependent changes in the viability of purified Cryptosporidium parvum oocysts by means of different excystation methods. Oocyst samples were 2 weeks to 12 months old and were treated with bile or sodium taurocholate, partly after pretreatment with hypochlorite. Pretreatment markedly enhanced the excystation of younger oocyst samples but did not increase excystation rates of 9 or 12-month-old oocysts. A cell culture-PCR assay was used as a second indicator for oocyst viability and was most consistent with excystation trials including oocyst pretreatment. In experiments aiming at the determination of the behaviour of the oocyst residual body during excystation, it could be demonstrated that it might be involved in this process.

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Authors and Affiliations

  1. Department of Biology, Namik Kemal University, 59030, Tekirdag, Turkey

    Sirri Kar & Nadim Yilmazer

  2. Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany

    Arwid Daugschies, Katja Dittmar & Berit Bangoura

  3. Department of Parasitology, Faculty of Veterinary Medicine, University of Ankara, 06110, Ankara, Turkey

    Ayse Cakmak

Authors
  1. Sirri Kar
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  2. Arwid Daugschies
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  3. Ayse Cakmak
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  4. Nadim Yilmazer
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  5. Katja Dittmar
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  6. Berit Bangoura
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Correspondence to Sirri Kar.

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Kar, S., Daugschies, A., Cakmak, A. et al. Cryptosporidium parvum oocyst viability and behaviour of the residual body during the excystation process. Parasitol Res 109, 1719–1723 (2011). https://doi.org/10.1007/s00436-011-2442-0

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  • DOI: https://doi.org/10.1007/s00436-011-2442-0

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