Abstract
In previous studies, cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) proteins induced specific humoral and cellular immune responses in susceptible and resistant mice in the absence of Trypanosoma cruzi infection with a significant induction of the Interferon-gamma (IFN-γ) production in those animals. In this follow-up paper, the immunostimulatory and protective effects of these proteins were evaluated by immunizing with CRA or FRA antigens, BALB/c and C57BL/6 mice and challenging with a T. cruzi (Y strain). Both proteins induced humoral response with high levels of IgG isotypes as well as cellular immunity with high levels of IFN-γ when compared to controls. However, the lymphocyte proliferative response was minimal. The survival rate at 30 days post-infection was significant in CRA (60%) or FRA (50%) - immunized BALB/c mice and CRA (83.3%) - immunized C57BL/6 mice. Taken as a whole these findings indicate that CRA and FRA are immunogenic and potentially important for protective immunity.
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Acknowledgements
We are grateful to Roni Evencio Araújo for his technical assistance . This investigation was supported by Fundação Oswaldo Cruz (Centro de Pesquisas Aggeu Magalhães and Biomanguinhos) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This research was partially supported by Bio-Manguinhos/FIOCRUZ and CNPq.
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Pereira, V.R.A., Lorena, V.M.B., Nakazawa, M. et al. Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection. Parasitol Res 96, 154–161 (2005). https://doi.org/10.1007/s00436-005-1336-4
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DOI: https://doi.org/10.1007/s00436-005-1336-4