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Resistance training restores the gene expression of molecules related to fat oxidation and lipogenesis in the liver of ovariectomized rats

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Abstract

Decreased levels of estrogen are associated with hepatic steatosis (HS), through changes in gene expression of molecules related to fat oxidation and lipogenesis. Both resistance training (RT) and endurance training (ET) prevent HS in ovariectomized (Ovx) rats. However, the molecular events associated with this process were only investigated for ET, but not for RT. Thus, the aim of this study was to investigate the effects of Ovx and RT on the gene expression of molecules related to fat oxidation and lipogenesis in the liver of rats. Sprague–Dawley adult female rats were grouped into four (n = 6 per group): sham-operated sedentary (Sham-Sed); Ovx sedentary (Ovx-Sed); sham-Rt and Ovx-Rt. A 10-week RT period, during which the animals climbed a 1.1-m vertical ladder with weights attached to their tails, was used. The sessions were performed three times a week, with 4–9 climbs and 8–12 dynamic movements per climb. Gene expression was analyzed by RT-PCR by the ∆∆Ct method. The estrogen deficiency associated with ovariectomy decreased the gene expression of molecules related to fat oxidation, carnitine palmitoyltransferase I (53%) and β-hydroxyacyl-CoA dehydrogenase (27%), and increased molecules related to lipogenesis, sterol regulatory element-binding protein-1c (106%), acetyl-CoA carboxylase (ACC) (72%) and stearoyl CoA desaturase-1 (109%). With the exception of ACC, the ovariectomy-induced changes in the expression of these molecules were restored by RT. The present results indicate that RT has important effects on the prevention of HS in Ovx animals, through changes in gene expression of molecules related to hepatic lipid metabolism.

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Acknowledgments

The authors thank Mr. José Carlos Lopes for the technical assistance in the laboratory. Financial support was provided by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and by the Exercise Physiology Laboratory of the Federal University of São Carlos (São Carlos, Brazil).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Physiological Sciences, Federal University of São Carlos, Flauzino Marques St, n. 67, ap: 14b, São Carlos, São Paulo, 13562-002, Brazil

    Mateus Moraes Domingos, Maria Fernanda Cury Rodrigues, Uliana Sbeguen Stotzer, Danilo Rodrigues Bertucci, Markus Vinicius Campos Souza, Diego Adorna Marine, Camila do Valle Gomes Gatto, Heloisa Sobreiro Selistre de Araújo & Sérgio Eduardo de Andrade Perez

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  1. Mateus Moraes Domingos
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  2. Maria Fernanda Cury Rodrigues
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  3. Uliana Sbeguen Stotzer
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  4. Danilo Rodrigues Bertucci
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  5. Markus Vinicius Campos Souza
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  6. Diego Adorna Marine
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  7. Camila do Valle Gomes Gatto
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  8. Heloisa Sobreiro Selistre de Araújo
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  9. Sérgio Eduardo de Andrade Perez
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Correspondence to Mateus Moraes Domingos.

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Communicated by Susan A. Ward.

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Domingos, M.M., Rodrigues, M.F.C., Stotzer, U.S. et al. Resistance training restores the gene expression of molecules related to fat oxidation and lipogenesis in the liver of ovariectomized rats. Eur J Appl Physiol 112, 1437–1444 (2012). https://doi.org/10.1007/s00421-011-2098-6

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  • DOI: https://doi.org/10.1007/s00421-011-2098-6

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