Abstract
A novel ”in vivo cryotechnique” with replica immunoelectron microscopy was developed for detecting caveolin localization on replica membranes prepared directly from living smooth muscle cells. After quick-freezing mouse duodenal walls by our ”in vivo cryotechnique”, the specimens were prepared for freeze-fracture and deep-etch replica membranes. Then they were treated with 5% SDS and 0.5% collagenase to keep some antigens on the replica membranes. The immunogold method could be used to clarify the localization of the caveolin antigen in relation to three-dimensional ultrastructures of living smooth muscle cells. Our new cryotechnique can provide native organization of functional molecules in living cells.
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Accepted: 7 October 1999
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Takayama, I., Terada, N., Baba, T. et al. ”In vivo cryotechnique” in combination with replica immunoelectron microscopy for caveolin in smooth muscle cells. Histochemistry 112, 443–445 (1999). https://doi.org/10.1007/s004180050426
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DOI: https://doi.org/10.1007/s004180050426