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Differential regulation of AQP2 trafficking in endosomes by microtubules and actin filaments

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Abstract

Vasopressin-induced trafficking of aquaporin-2 (AQP2) water channels in kidney collecting duct cells is critical to regulate the urine concentration. To better understand the mechanism of subcellular trafficking of AQP2, we examined MDCK cells expressing AQP2 as a model. We first performed double-immunolabeling of AQP2 with endosomal marker proteins, and showed that AQP2 is stored at a Rab11-positive subapical compartment. After the translocation to the plasma membrane, AQP2 was endocytosed to EEA1-positive early endosomes, and then transferred back to the original Rab11-positive compartment. When Rab11 was depleted by RNA interference, retention of AQP2 at the subapical storage compartment was impaired. We next examined the role of cytoskeleton in the AQP2 trafficking and localization. By the treatment with microtubule-disrupting agent such as nocodazole or colcemid, the distribution of AQP2 storage compartment was altered. The disruption of actin filaments with cytochalasin D or latrunculin B induced the accumulation of AQP2 in EEA1-positive early endosomes. Altogether, our data suggest that Rab11 and microtubules maintain the proper distribution of the subapical AQP2 storage compartment, and actin filaments regulate the trafficking of AQP2 from early endosomes to the storage compartment.

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Acknowledgements

We wish to thank Ms. Y. Takahashi-Tajika and Ms. S. Fukushima for secretarial and technical assistance. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Yuki Tajika, Toshiyuki Matsuzaki, Takeshi Suzuki, Abdushukur Ablimit, Takeo Aoki, Haruo Hagiwara & Kuniaki Takata

  2. Department of Homeostasis Medicine and Nephrology, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan

    Michio Kuwahara & Sei Sasaki

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  1. Yuki Tajika
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  2. Toshiyuki Matsuzaki
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  3. Takeshi Suzuki
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  4. Abdushukur Ablimit
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  5. Takeo Aoki
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  9. Kuniaki Takata
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Correspondence to Kuniaki Takata.

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Tajika, Y., Matsuzaki, T., Suzuki, T. et al. Differential regulation of AQP2 trafficking in endosomes by microtubules and actin filaments. Histochem Cell Biol 124, 1–12 (2005). https://doi.org/10.1007/s00418-005-0010-3

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  • DOI: https://doi.org/10.1007/s00418-005-0010-3

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