Abstract
Small, monodisperse nanogels (∼50-nm radius) were synthesized by free-radical precipitation polymerization and were characterized using a suite of light scattering and chromatography methods. Nanogels were synthesized with either N-isopropylacrylamide or N-isopropylmethacrylamide as the main monomer, with acrylic acid or 4-acrylamidofluorescein as a comonomer and N,N′-methylenebis(acrylamide) as a cross-linker. By varying the surfactant and initiator concentrations, particle size was controlled while maintaining excellent monodispersity. An amine-containing shell was added to these core particles to facilitate subsequent bioconjugation. Successful conjugation of folic acid to the particles was demonstrated as an example of how such materials might be employed in a targeted drug delivery system.
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Abbreviations
- NIPAm:
-
N-isopropylacrylamide
- LCST:
-
lower critical solution temperature
- NIPMAm:
-
N-isopropylmethacrylamide
- AAc:
-
acrylic acid
- EPR:
-
enhanced permeability and retention
- RES:
-
reticuloendothelial system
- APS:
-
ammonium persulfate
- SDS:
-
sodium dodecyl sulfate
- BIS:
-
N,N′-methylenebis(acrylamide)
- APMA:
-
N-(3-aminopropyl) methacrylamide hydrochloride
- EDC:
-
1-ethyl-3-methyl-(3-dimethylaminopropyl)carbodiimide
- DMSO:
-
dimethyl sulfoxide
- AFA:
-
4-acrylamidofluorescein
- MALLS:
-
multi-angle laser light scattering
- AFFF:
-
asymmetric field flow fractionation
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Acknowledgements
The authors acknowledge financial support from the Department of Health and Human Services (1 R21 EB006499-01) and the Emory-Georgia Tech Nanotechnology Center for Personalized and Predictive Oncology (5-40256-G11).
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Blackburn, W.H., Lyon, L.A. Size-controlled synthesis of monodisperse core/shell nanogels. Colloid Polym Sci 286, 563–569 (2008). https://doi.org/10.1007/s00396-007-1805-7
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DOI: https://doi.org/10.1007/s00396-007-1805-7