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Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro

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Abstract

Patchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 30973693, No. 81273616) and the Research Fund for the Doctoral Program of Higher Education of China (No. 20104401110003).

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou, China

    Xian-Lin Wu, Jia Chen, Bin Yu, Jin-Xiong He, Cong-Qi Dai, Zhou Chang, You-Ping Wang & Xiao-Yin Chen

  2. Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing, China

    Da-Hong Ju

  3. Department of Internal Medicine, Nancheng People’s Hospital, Dongguan, China

    Kang-Li Liu

  4. Department of Microbiology and Immunology, Medical College of Jinan University, Guangzhou, China

    Sha Wu

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  1. Xian-Lin Wu
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  2. Da-Hong Ju
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  9. Zhou Chang
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  11. Xiao-Yin Chen
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Correspondence to Xiao-Yin Chen.

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Xian-Lin Wu and Da-Hong Ju contributed equally to this article.

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Wu, XL., Ju, DH., Chen, J. et al. Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro. Curr Microbiol 67, 431–436 (2013). https://doi.org/10.1007/s00284-013-0381-y

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  • DOI: https://doi.org/10.1007/s00284-013-0381-y

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