Abstract
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling conditions. The process of optimizing a multiplex PCR reaction in order to obtain a well-balanced set of amplicons can be time-consuming and labor-intensive. The rapid separation and quantitation capabilities of capillary electrophoresis make it an efficient technique to help in the multiplex PCR optimization process.
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Received: 7 September 2000 / Revised: 30 October 2000 / Accepted: 4 November 2000
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Butler, J., Ruitberg, C. & Vallone, P. Capillary electrophoresis as a tool for optimization of multiplex PCR reactions. Fresenius J Anal Chem 369, 200–205 (2001). https://doi.org/10.1007/s002160000641
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DOI: https://doi.org/10.1007/s002160000641