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Chromatographic and mass spectrometric fingerprinting analyses of Angelica sinensis (Oliv.) Diels-derived dietary supplements

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Abstract

Angelica sinensis (Oliv.) Diels (“Danggui” in Chinese) is one of the most commonly used traditional Chinese medicines. It has been used to invigorate blood circulation for the treatment of anemia, hypertension, chronic bronchitis, asthma, rheumatism, and cardiovascular diseases. There are a number of A. sinensis-derived dietary supplements in the US markets. However, no study have been conducted to investigate the quality of these dietary supplements. In this paper, high-performance liquid chromatographic and flow-injection mass spectrometric fingerprints were both evaluated to assess the consistency of A. sinensis-derived dietary supplements. Similarity analysis was carried out on the high-performance liquid chromatographic (HPLC) fingerprints. Meanwhile, principal component analysis (PCA) was performed on the data obtained from flow-injection mass spectrometric (FIMS) fingerprints, which can analyze each sample in 2 min, compared with 30 min required for the chromatographic fingerprint. Both methods show significant chemical differences between samples that may be due to differences in growing locations, growing conditions, harvesting times, and/or botanical processing. The loading plots obtained from PCA singled out the discriminatory ions that were responsible for chemical differences of A. sinensis-derived dietary supplements.

In the present study, HPLC and flow-injection mass spectrometric fingerprints as well as chemometrics were applied to assess the consistency of A. sinensis-derived dietary supplements from U.S. markets in order to understand the variability of the products and to provide useful information with customers.

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Acknowledgment

This research is supported by the Agricultural Research Service of the US Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health.

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Authors and Affiliations

  1. Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, 20705, USA

    Yang Zhao, Jianghao Sun & Pei Chen

  2. Department of Nutrition and Food Science, University of Maryland, College Park, MD, 20742, USA

    Liangli Lucy Yu

Authors
  1. Yang Zhao
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  2. Jianghao Sun
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  3. Liangli Lucy Yu
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  4. Pei Chen
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Correspondence to Pei Chen.

Additional information

Published in the topical collection Functional Foods and Dietary Supplements with guest editors Melissa M. Phillips and Catherine A. Rimmer.

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Zhao, Y., Sun, J., Yu, L.L. et al. Chromatographic and mass spectrometric fingerprinting analyses of Angelica sinensis (Oliv.) Diels-derived dietary supplements. Anal Bioanal Chem 405, 4477–4485 (2013). https://doi.org/10.1007/s00216-012-6668-1

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  • DOI: https://doi.org/10.1007/s00216-012-6668-1

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