Skip to main content
SpringerLink
Log in

Characterization of steroidal saponins in saponin extract from Paris polyphylla by liquid chromatography tandem multi-stage mass spectrometry

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Rhizoma Paridis saponins are bioactive steroidal saponins derived from Paris polyphylla. Optimization of the ionization process was performed with electrospray ionization tandem mass spectrometry in both positive and negative-ion modes. Negative-ion ESI was adopted for generation of the precursor deprotonated molecules to achieve the best ionization sensitivity for the analytes. Positive ionization was used to choose the most abundant fragment ion. Furthermore, according to the characteristic fragmentation behavior of known steroidal saponins isolated from this plant (polyphyllin D, formosanin C, gracillin, Paris H, Paris VII, and dioscin), 23 constituents were structurally characterized on the basis of their retention time and ESI analyses, including four pairs of naturally occurring isomers. Five of these 23 constituents were new compounds. The analytical method of LC–MSn in positive and negative-ion modes has been developed for the direct structural elucidation of steroid saponins of this kind in plant extracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

Abbreviations

Ara:

α-l-arabinofuranosyl

FC:

formosanin C

Glc:

β-d-glucopyranosyl

PD:

polyphyllin D

PPY:

Paris polyphylla var. yunnanensis (Fr.) Hand.-Mazz

Rha:

α-l-rhamnopyranosyl

RPS:

Rhizoma Paridis saponins

References

  1. Yan LL, Zhang YJ, Gao WY, Man SL (2008) China Journal of Chinese Materia Medica 33:2057

    CAS  Google Scholar 

  2. Wang Y, Gao WY, Zhang TJ, Guo YQ (2007) Chin Chem Lett 18:548

    Article  Google Scholar 

  3. Wang Y, Gao WY, Yuan LC, Liu XQ, Wang SJ, Chen C (2007) Chinese Traditional and Herbal Drugs 38:17

    Google Scholar 

  4. Wang Y, Gao WY, Yan LL (2007) Zhongguo Zhong Yao Za Zhi 32:1425

    CAS  Google Scholar 

  5. Luo M (2004) Zhong Yao Cai 27:478

    Google Scholar 

  6. Zhang LT, Zuo YT, Gao WY, Zhang FY, Chen HX, Duan HQ (2007) Chinese Traditional and Herbal Drugs 38:422

    CAS  Google Scholar 

  7. Liu Y, Li J, Yu S, Abliz Z, Liu Y, Qu J, Liu J, Hu Y (2008) Anal Chim Acta 611:187

    Article  CAS  Google Scholar 

  8. Cheng L, Zhang M, Zhang P, Song Z, Ma Z, Qu H (2008) Rapid Commun Mass Spectrom 22:3783

    Article  CAS  Google Scholar 

  9. Cai F, Sun L, Gao S, Yang Y, Yang Q, Chen W (2008) J Pharm Biomed Anal 48:1411

    Article  CAS  Google Scholar 

  10. Zheng Z, Zhang W, Kong L, Liang M, Li H, Lin M, Liu R, Zhang C (2007) Rapid Commun Mass Spectrom 21:279

    Article  CAS  Google Scholar 

  11. Zehl M, Pittenauer E, Jirovetz L, Bandhari P, Singh B, Kaul VK, Rizzi A, Allmaier G (2007) Anal Chem 79:8214

    Article  CAS  Google Scholar 

  12. Chapagain BP, Wiesman Z (2007) Phytochem Anal 18:354

    Article  CAS  Google Scholar 

  13. Li B, Abliz Z, Tang M, Fu G, Yu S (2006) J Chromatogr A 1101:53

    Article  CAS  Google Scholar 

  14. Cui M, Song F, Zhou Y, Liu Z, Liu S (2000) Rapid Commun Mass Spectrom 14:1280

    Article  CAS  Google Scholar 

  15. Lin S, Wang D, Yang D, Yao J, Tong Y, Chen J (2007) Anal Chim Acta 599:98

    Article  CAS  Google Scholar 

  16. Li R, Zhou Y, Wu Z, Ding L (2006) J Mass Spectrom 41:1

    Article  Google Scholar 

  17. Lu Y, Luo J, Xu D, Huang X, Kong L (2008) Rapid Commun Mass Spectrom 22:2447

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by a grant 07JCZDJC05400 from the Tianjin Municipal Science and Technology Commission (Tianjin, China) and a grant 30873378 from National Natural Science Foundation of China.

Author information

Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin, 300072, China

    Shuli Man, Wenyuan Gao, Xinghua Jin, Chaoyi Ma & Xianxiao Huang

  2. Tianjin University of Traditional Chinese Medicine, Tianjin, China

    Yanjun Zhang & Qingyun Li

Authors
  1. Shuli Man
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenyuan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanjun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xinghua Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chaoyi Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xianxiao Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Qingyun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wenyuan Gao.

Additional information

Yanjun Zhang and Wenyuan Gao contributed equally to this paper.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Man, S., Gao, W., Zhang, Y. et al. Characterization of steroidal saponins in saponin extract from Paris polyphylla by liquid chromatography tandem multi-stage mass spectrometry. Anal Bioanal Chem 395, 495–505 (2009). https://doi.org/10.1007/s00216-009-2987-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-009-2987-2

Keywords

Search

Navigation