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An easy and rapid method to determine aristolochic acids I and II with high sensitivity

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Abstract

Aristolochic acid (AA), a natural component in some Chinese medicinal plants, is nephrotoxic and carcinogenic, and is involved in a specific type of renal fibrosis, called Chinese herbs nephropathy (CHN). In this paper, we report the electrochemistry of AA and a novel method to detect the species based on the electrochemical studies. The detection limit is estimated to be 1.0×10-8 M with a linear range from 5.0×10-8 M to 1.3×10-6 M. Meanwhile, this method is applicable in detection in real samples, such as Caulis Aristolochiae Manshuriensis (CAM), Radix Aristolochiae (RA), Fructus Aristolochiae (FA) and Radix Stephaniae Tetrandrae (RST). The concentration of AA in the CAM sample was 3.50±0.12×10-4 M. Consistent results have been obtained from both the electrochemical approach described here and the previously reported HPLC method.

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References

  1. Vanherweghem JL (2002) M S-Med Sci 18:1095–1101

  2. Cosyns JP (2003) Drug Safety 26:33–48

    CAS  Google Scholar 

  3. Cosyns JP, Goebbels RM, Liberton V (1998) Arch Toxicol 72:738–743

    Article  CAS  Google Scholar 

  4. Nortier JL, Vanherweghem JL (2002) Toxicology 181:577–580

    Article  Google Scholar 

  5. Stiborova M, Schmeiser HH, Breuer A (2001) Gen Physiol Biophys 20:267–279

    CAS  Google Scholar 

  6. Kohara A, Suzuki T, Honma M (2002) Mutat Res Genet Toxicol Environ Mutagenesis 515:63–72

    Article  CAS  Google Scholar 

  7. Lord GM, Tagore R, Cook T, Gower P, Pusey CD (1999) Lancet 354:481–482

    Article  CAS  Google Scholar 

  8. Chang CH, Wang YM, Yang AH (2001) Am J Nephrol 21:441–448

    Article  CAS  Google Scholar 

  9. Ioset JR, Raoelison GE, Hostettmann K (2003) Food Chem Toxicol 41:29–36

    Article  CAS  Google Scholar 

  10. Hashimoto K, Higuchi M, Makino B, Sakakibara I, Kubo M, Komatsu Y, Maruno M, Okada M (1999) J Ethnopharmacol 64:185–189

    Article  CAS  Google Scholar 

  11. Schaneberg BT, Applequist WL, Khan IA (2002) Pharmazie 57:686–689

    CAS  Google Scholar 

  12. Tsai TH, Chou CJ, Lin LC, Tsai W J, Chen CF (1993) J Liq Chromatogr 16:1173 −1182

    CAS  Google Scholar 

  13. Vanherweghem JL, Depierreux M, Tielemans C (1993) Lancet 341:387–391

    CAS  PubMed  Google Scholar 

  14. Mix DB, Guinaudeau H, Shamma M (1982) J Nat Prod 45:657–666

    CAS  Google Scholar 

  15. Fan C-H, Li G-X, Zhu J-Q, Zhu D-X (2000) Anal Chim Acta 423:95–100

    Article  CAS  Google Scholar 

  16. Kastening B, Zuman P (1972) Prog in Polarogr 3:195–286

    CAS  Google Scholar 

  17. Zou H, Yuan Z-B (1997) J Anal Sci l3:316–318

    Google Scholar 

  18. Lee TY, Wu ML, Deng JF, Hwang DF (2001) J Chromatogr B 766:169–174

    Google Scholar 

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Acknowledgements

We greatly appreciate the support of the National Natural Science Foundation, and the Ministry of Education, P. R. China for this research.

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Authors and Affiliations

  1. Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, P. R. China

    Ziyi Sun, Lifang Liu, Xiaofeng Zheng, Chunhai Fan & Genxi Li

  2. Department of Pharmacognosy, China Pharmaceutical University, 210009, Nanjing, P. R. China

    Qiang Wang

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  1. Ziyi Sun
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  2. Lifang Liu
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  3. Xiaofeng Zheng
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  4. Chunhai Fan
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  5. Qiang Wang
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  6. Genxi Li
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Correspondence to Genxi Li.

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Sun, Z., Liu, L., Zheng, X. et al. An easy and rapid method to determine aristolochic acids I and II with high sensitivity. Anal Bioanal Chem 378, 388–390 (2004). https://doi.org/10.1007/s00216-003-2209-2

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  • DOI: https://doi.org/10.1007/s00216-003-2209-2

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