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Protective effect of resveratrol in endotoxemia-induced acute phase response in rats

  • Organ Toxicity and Mechanisms
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Abstract

Lipopolysaccharide (LPS), a glycolipid component of the cell wall of gram-negative bacteria can elicit a systemic inflammatory process leading to septic shock and death. Acute phase response is characterized by fever, leucocytosis, thrombocytopenia, altered metabolic responses and redox balance by inducing excessive reactive oxygen species (ROS) generation. Resveratrol (trans-3,5,4′ trihydroxystilbene) is a natural polyphenol exhibiting antioxidant and anti-inflammatory properties. We investigated the protective effect of resveratrol on endotoxemia-induced acute phase response in rats. When acutely administered by i.p. route, resveratrol (40 mg/kg b.w.) counteracted the effect of a single injection of LPS (4 mg/kg b.w.) which induced fever, a decrease in white blood cells (WBC) and platelets (PLT) counts. When i.p. administered during 7 days at 20 mg/kg per day (subacute treatment), resveratrol abrogated LPS-induced erythrocytes lipoperoxidation and catalase (CAT) activity depression to control levels. In the plasma compartment, LPS increased malondialdehyde (MDA) via nitric monoxide (NO) elevation and decreased iron level. All these deleterious LPS effects were reversed by a subacute resveratrol pre-treatment via a NO independent way. Resveratrol exhibited potent protective effect on LPS-induced acute phase response in rats.

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Abbreviations

CAT:

Catalase

LPS:

Lipopolysaccharide

MDA:

Malondialdehyde

NO:

Nitric oxide

NOS:

Nitric oxide synthase

PLT:

Platelets

ROS:

Reactive oxygen species

RVT:

Resveratrol

WBC:

White blood cells

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Acknowledgment

Financial support of the Tunisian Ministry of “Enseignement Supérieur, Recherche Scientifique et Technologie” is gratefully acknowledged.

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Authors and Affiliations

  1. Département des Sciences de la Vie, Faculté des Sciences de Bizerte, UR Ethnobotanie & Stress Oxydant, 7021, Zarzouna, Tunisia

    Hichem Sebai, Ezzedine Aouani & Néziha Ghanem-Boughanmi

  2. Département des Sciences de la Vie, Faculté des Sciences de Bizerte, Laboratoire de Biosurveillance de l’Environnement (LBE), 7021, Zarzouna, Tunisia

    Mossadok Ben-Attia & Mamane Sani

  3. Faculté de Médecine, Timone, INSERM UMR-911, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France

    Hichem Sebai

Authors
  1. Hichem Sebai
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  2. Mossadok Ben-Attia
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  3. Mamane Sani
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  4. Ezzedine Aouani
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  5. Néziha Ghanem-Boughanmi
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Correspondence to Mossadok Ben-Attia.

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Sebai, H., Ben-Attia, M., Sani, M. et al. Protective effect of resveratrol in endotoxemia-induced acute phase response in rats. Arch Toxicol 83, 335–340 (2009). https://doi.org/10.1007/s00204-008-0348-0

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  • DOI: https://doi.org/10.1007/s00204-008-0348-0

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