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Polymorphism of GSTM1 and GSTT1 genes in bladder cancer: a study from North India

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Abstract

The present study was conducted (1) to examine whether the GSTT1- and GSTM1-null genotypes are risk factors for bladder cancer, and (2) to study possible association of tobacco usage and age strata with genotype of these patients. This case control study was undertaken over a period of 19 months and included 106 bladder cancer patients and 182 controls; both patients and controls originated from northern part of India. The GSTT1 and GSTM1 genotypes were identified by multiplex PCR in peripheral blood DNA samples. Genotype frequencies among patients and controls were assessed and the association of the genotypes with smoking habits and gender of the patients were statistically determined by the χ2 test. Frequencies of null genotypes in GSTT1 and GSTM1, were 16% (29/182) and 30% (54/182), respectively, in control individuals. The frequencies of GSTT1- and GSTM1-null genotypes in bladder cancer patients were 26% (28/106) and 40% (42/106), respectively. In conclusion, our study demonstrated that the null genotypes of GSTT1 and GSTM1 were substantially at higher risk for bladder carcinoma compared to the normal healthy controls. The GSTT1- and GSTM1-null genotypes did not show significant association with tobacco usage in bladder cancer patients. However, the null genotypes were statistically significant in female relative to male bladder cancer patients.

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Acknowledgements

The authors are grateful to the Director, Sanjay Gandhi PGIMS for providing the necessary facilities. One of the authors (Daya Shankar Lal Srivastava) is grateful to Council of Scientific and Industrial Research, New Delhi, for the award of a research fellowship.

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Authors and Affiliations

  1. Department of Urology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Raebareli Road, 226014, Lucknow, Uttar Pradesh, India

    Daya Shankar Lal Srivastava, Anant Kumar & Rama Devi Mittal

  2. Department of Genetics, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, 226014, Lucknow, Uttar Pradesh, India

    Balraj Mittal

Authors
  1. Daya Shankar Lal Srivastava
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  2. Anant Kumar
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  3. Balraj Mittal
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  4. Rama Devi Mittal
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Correspondence to Rama Devi Mittal.

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Srivastava, D.S.L., Kumar, A., Mittal, B. et al. Polymorphism of GSTM1 and GSTT1 genes in bladder cancer: a study from North India. Arch Toxicol 78, 430–434 (2004). https://doi.org/10.1007/s00204-004-0559-y

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  • DOI: https://doi.org/10.1007/s00204-004-0559-y

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