Abstract
It is not known why hypothyroidism predisposes the ovary to develop cystic follicles in hCG-treated rats. This study examined the effect of TSH on steroidogenesis in cultured granulosa cells. Granulosa cells were isolated from the ovaries of hCG-treated Long-Evans rats. Cells were cultured in medium with either 0 or 100nm testosterone or 25(OH)cholesterol and treated with 0, 2.5, 10 or 20 ng/ml of TSH. TSH reduced 3β-HSDI activity in these cells to 12% of control. Progesterone secretion decreased by 72% and estradiol secretion was non-detectable during the second day of treatment with 20 ng/ml of TSH. We conclude that TSH is capable of regulating the secretion of progesterone and estradiol by cultured granulosa cells.
Similar content being viewed by others
References
Adams, W.C. & Leathern, J.H. (1965).Endocrinology,76, 1041–1046.
Akamizu, T., Ikuyama, S., Saji, M., Kosugi, S., Kozak, C., McBride, O.W. & Kohn, L.D. (1990).Proc. Natl. Acad. Sci. USA,87, 5677–5681.
Armstrong, D.T. & Dorrington, J.H. (1976).Endocrinology,99, 1411–1414.
Brown-Grant, K., Dutton, A. & Ter Haar, M.B. (1977).J. Endocr.,72, 33P-34P.
Bruot, B.C. (1986).Proc. Soc. Expt. Biol Med.,184, 206–210.
Cooke, P.S. & Meisami, E. (1991).Endocrinology,129, 237–243.
Davies, T.F., Smith, B.R. & Hall, R. (1978).Endocrinology,103, 6–10.
De Silva, M., Conley, F.A. & Lee, C.S. (1991).Biol. Reprod.,44(Supp.I), 67.
Duleba, A.J., Takahashi, H. & Moon, Y.S. (1983).Steroids,42(3), 321–330.
Erickson, L.D., Rizza, S.A., Bergert, E.R., Charlesworth, M.C., McCormick, D.J. & Ryan, R.J. (1990).Endocrinology,126, 2555–2560.
Fanjul, L.F. Déniz, A., Quintana, J., Santana, P., Gonzalez, J., Estevez, F. & Ruiz de Galarreta, C.M. (1992).Biochem. Biophy. Res. Comm.,188(1), 198–203.
Gerhard, I., Becker, T., Eggert-Kruse, W., Klinga, K. & Runnebaum, B. (1991).Human Reproduction,6(3), 338–345.
Gerhard, I., Eggert-Kruse, W., Merzoug, K., Klinga, K. & Runnebaum, B. (1990).Gynecol. Endocrinol.,5(1), 15–32.
Grasso, P. & Crisp, T.M. (1985a).Endocrinology,116, 319–327.
Grasso, P. & Crisp, T.M. (1985b).Biol Repro.,32, 935–945.
Haynes, R.C. Jr. (1985).Goodman and Gilman’s The pharmacological basis of therapeutics. Gilman, A.G., Goodman, L.S., Rail, T.W. & Murad, F. (eds). Macmillan Publishing Company: New York. pp. 1401–1403.
Hudson, K.E., Wickings, E.J. & Hillier, S.G. (1987).J. Steroid. Biochem.,28(3), 267–272.
Hutson, J.C. (1978).Am. J. Anat.,151, 55–70.
Hutson, J.C. & Stocco, D.M. (1981).Biol. Reprod.,25, 303–306.
Hutson, J.C. & Stocco, D.M. (1978).Biol. Reprod.,19, 768–772.
Labarca, C. & Paigen, K. (1980).Anal. Biochem.,102(2), 344–352.
Latman, N.S., Kishore, V. & Bruot, B.C. (1986).Arthritis Rheum.,29, 411–414.
Leathern, J.H. (1958).Anat. Rec.,131, 487–499.
Lee, M.T., Bruot, B.C. & Adams, W.C. (1986).Biol. Repro.,35, 542–548.
Lindsay, A.N., Voorhess, M.L. & MacGillivray, M.H. (1980).Am. J. Dis. Child.,134, 588–592.
Maruo, T., Hayashi, M., Matsuo, H., Yamamoto, T., Okada, H. & Mochizuki, M. (1987).Endocrinology,121, 1233–1241.
Mita, M., Price, J.M. & Hall, P.F. (1982).Endocrinology,110, 1535–1541.
Moon, Y.S., Duleba, A.J. & Takahashi, H. (1984).Biochem. Biophys. Research Comm.,119(2), 694–699.
Nalbandov, A.V. (1952).Fertil. Steril.,3, 100–120.
Néauport, C. & Emmerich, E. (1981).CR. Soc. Seances. Soc. Biol. Fil.,175,(2), 140–143.
Quirk, S.M., Hilbert, J.L. & Fortune, J.E. (1986).Endocrinology,118, 2402–2410.
Scommegna, A. (1965).Fertil. Steril.,16, 571–578.
Stalvey, J.R.D. & Clavey, S.M. (1992).J. Androl.,13(1), 93–99.
Stalvey, J.R.D. & Payne, A.H. (1984).Endocrinology,115, 1500–1505.
Trokoudes, K.M., Sugenoya, A., Hazani, E., Row, V.V. & Volpé, R. (1979).J. Clin. Endocrinol. Metab.,48, 919–923.
Wright, K., Collins, D.C. & Preedy, J.R.K. (1978).J. Clin. Endo. Metab.,47, 1084–1091.
Young, S.L. & Melner, M.H. (1989).Steroids,54(6), 583–591.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mehendale, R.G., Bruot, B.C. Thyroid stimulating hormone inhibits rat granulosa cell steroidogenesis in primary culture. Endocr 3, 215–220 (1995). https://doi.org/10.1007/BF02994446
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02994446