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Low frequency loss of heterozygosity in theBRCA1 region in Japanese sporadic breast cancer

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Abstract

To investigate the possible involvement of theBRCA1 gene in Japanese sporadic breast cancer we have analyzed loss of heterozygosity (LOH) at theBRCA1 region (17q12-q22) in 101 sporadic breast cancers using 5 microsatellite markers, such asD17S250, D17S846, D17S855, D17S579, andNME1. The frequency of LOH in each markers was 14.9% (11/74), 11.9% (8/67), 14.3% (12/84), 5.3% (4/75), and 13.3% (9/ 68), respectively. The incidence of LOH in at least one of 5 markers was 23.8% (24/ 101). The LOH at theBRCA1 region did not significantly correlate with clinicopathologic factors. Despite using a microdissection method, the LOH frequency in our series was relatively lower than other reports, especially in Western countries. We suggest that theBRCA1 gene might play only a limited role as a tumor suppressor gene in the majority of Japanese sporadic breast cancers.

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Abbreviations

LOH:

Loss of heterozygosity

PCR:

Polymerase chain reaction

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Authors and Affiliations

  1. Second Department of Surgery, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, 467, Nagoya, Japan

    Toshinari Yamashita, Hirotaka Iwase, Hiroji Iwata, Kazuko Ito, Tatsuya Toyama, Yasuo Hara & Shunzo Kobayashi

  2. Department of Clinical Research, National Sapporo Hospital, Japan

    Hiroko Yamashita

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  1. Toshinari Yamashita
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  2. Hirotaka Iwase
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  3. Hiroko Yamashita
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  4. Hiroji Iwata
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  7. Yasuo Hara
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Yamashita, T., Iwase, H., Yamashita, H. et al. Low frequency loss of heterozygosity in theBRCA1 region in Japanese sporadic breast cancer. Breast Cancer 3, 167–172 (1996). https://doi.org/10.1007/BF02966980

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  • DOI: https://doi.org/10.1007/BF02966980

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