Summary
109 malignant lymphomas were surveyed by Southern blot analysis and polymerase chain reaction (PCR) for Epstein-Barr virus (EBV) DNA and compared with 16 examples of non-neoplastic lymphadenopathy and 4 normal thymuses. In specimens positive by the method of Southern and PCR, in situ hybridization studies were performed on formalin-fixed, paraffin-embedded sections. By Southern blot analysis, two of seven Hodgkin’s disease samples (29%) (one of mixed cellularity and the other of lymphocyte predominance type), three of 56 B-cell lymphomas (5.6%) and five of 46 Tcell lymphomas (11 %) demonstrated EBV DNA. However, the 16 examples of lymphadenitis and the 4 normal thymuses showed no EBV DNA. With PCR, EBV DNA was identified in one B-cell lymphoma, nine T-cell lymphomas, ten lymphadenitis specimens and two of the normal thymus, in addition to the positive specimens determined by the Southern blotting method. These results indicate that the presence of EBV DNA is not related to lymphoid malignancy, but enhancement of the DNA is demonstrated in some neoplastic conditions. By in situ hybridization, EBV genomes were not detected in all PCR-positive cases, but only in those positive by Southern blot analysis.
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References
Bayliss GJ, Hans Wolf (1980) Epstein-Barr virus-induced cell fu-sion. Nature 287:164–165
Brown MA, Liu C, Garcia CR, Wang YF, Griffith A, Sparkes RS, Calame KL (1986) Clonal origins of lymphoproliferative disease induced by Epstein-Barr virus. J Virol 58:975–978
Choppuis BB, Müller H, Stutte J, Hey MM, Hübner K, Müller-Hermelink HK (1990) Identification of EBV-DNA in lymph nodes from patients with lymphadenopathy and lymphomas associated with AIDS. Virchows Arch [B] 56:199–205
Cleary ML, Nalesnik MA, Shearer WT, Sklar J (1988) Clonal anal-ysis of transplant-associated lymphoproliferations based on the structures of genomic termini of the Epstein-Bar virus. Blood 72:349–352
Epstein MA, Achong BG (1979) Introduction: discovery and gen-eral biology of the virus. In: Epstein MA, Achong BG (eds) The Epstein-Barr virus. Springer, Berlin Heidelberg New York, pp 1–22
Fingeroth JD, Weiss JJ, Tedder TF, Strominger JL, Biro PA, Fear-on DT (1984) Epstein-Barr virus receptor of human B lympho-cytes is the C3d receptor CR2. Proc Natl Acad Sci 81:4510–4514
Grufferman S, Elizabeth D (1984) Epidemiology of Hodgkin’s dis-ease. Epidemiol Rev 6:76–106
Hanto DW, Frizzera G, Gajl-Peczalska KJ, Simmons RL (1985) Epstein-Barr virus, immunodeficiency, and B cell lymphoproli-feration. Transplantation 35:461–472
Henle W, Diehl V, Kohn G, ZurHausen H, Henle G (1967) Herpes-type virus and chromosone marker in normal leukocytes after growth with irradiated Burkitt’s cells. Science 157:1064–1065
Hirose Y, Shimizu S, Takiguchi T, Sugai S, Konda S (1984) Elec-tron microscopic study of cell lines established from adult T-cell leukemia-coexistence of adult T-cell leukemia virus and Ep-stein-Barr virus. Jpn J Clin Oncol 14:585–594
Ishihara S, Tawa A, Yumura-Yagi K, Murata M, Hara J, Yabuchi H, Hirai K, Kawa-Ha K (1989) Clonal T-cell lymphoprolifera-tion containing Epstein-Barr (EB) virus DNA in a patient with chronic active EB virus infection. Jpn J Cancer Res 80:99–101
Lenoir GM, Philip T, Sohier RL (1984) Burkitt-type lymphoma: EBV association and cytogenetic markers in cases from various geographic locations. In: Margrath IT, O’Conor GT, Ramot B (eds) Pathogenesis of leukemias and lymphomas: environ-mental influences. Raven Press, New York, pp 283–295
Leyvraz S, Henle W, Chahinian P, Perlmann C, Klein G, Gordon R, Rosenblum M, Holland JF (1985) Association of Epstein-Barr virus with thymic carcinoma. N Engl J Med 312:1296–1299
Mueller N (1987) Epidemiologic studies assessing the role of the Epstein-Barr virus in Hodgkin’s disease. Yale J Biol Med 60:321–332
Penn I (1986) Cancer is a complication of severe immunosuppres-sion. Surg Gynecol Obstet 162:603–610
Raab-Traub N, Flynn K (1986) The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular prolifera-tion. Cell 47:883–889
Saemunden AK, Albeck H, Hansen JPH (1982) Epstein-Barr virus in nasopharyngeal and salivary gland carcinomas in Greenland Eskimoes. Br J Cancer 46:721–728
Saito I, Servenius B, Compton T, Fox RI (1989) Detection of Epstein-Barr virus DNA by polymerase chain reaction in blood and tissue biopsies from patients with Sjogren’s syndrome. J Exp Med 169:2191–2198
Saw D, Lau WH, Ho JHC, Chan JKC, Ng CS (1986) Malignant lymphoepithelial lesion of the salivary gland. Hum Pathol 17:914–923
Sixbey JW, Davis DS, Young LS, Hutt-Fletcher L, Tedder TF, Rickinson AB (1987) Human epithelial cell expression of an Epstein-Barr virus receptor. J Gen Virol 68:805–811
Staal SP, Ambinder R, Beschorner WE, Hayward GS, Mann R (1989) A survey of Epstein-Barr virus DNA in lymphoid tissue. AJCP 91:l-5
Suchi T, Tazima K, Nanba K, Wakasa H, Mikata A, Kikuchi M, Mori S, Wanabe S, Mohri N, Shamoto M, Harigaya K, Itagaki J (1979) Some problems on the histopathological diag-nosis of Non-Hodgkin’s malignant lymphoma -A proposal of a new type. Acta Pathol Jpn 29:755–776
Tosato G (1987) The Epstein-Barr virus and the immune system. Adv Cancer Res 49:75–125
Uhara H, Sato Y, Mukai K, Akao I, Matsuno Y, Furuya S, Hos-hikawa T, Shimosato Y, Saida T (1990) Detection of Epstein-Barr virus DNA in Reed-Sternberg cells of Hodgkin’s disease using the polymerase chain reaction and in in situ hybridization. Jpn J Cancer Res 81:272–278
Weiss L, Movahed LA (1989) In situ demonstration of Epstein-Barr viral genomes in viral-associated B cell lymphoproliferations. AJP 134:651–659
Weiss LM, Movahed LA, Warnke RA, Sklar J (1989) Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodg-kin’s disease. N Engl J Med 320:502–506
Weiss L, Strickler JG, Warnke RA, Purtilo DT, Sklar J (1987) Epstein-Barr viral DNA in tissues of Hodgkin’s disease. AJP 129:86–91
Yoshida T, Kikuchi M, Ooshima K, Takeshita M, Kimura N, Kozuru M, Satoh H (1989) ATLA-positive adult T-cell leuke-mia/lymphoma with no monoclonal proviral DNA. A clinico-pathological and immunological study. Cancer 64:2515–2524
Young LS, Clark D, Sixbey JW, Rickinson AB (1986) Epstein-Barr virus receptors on human pharyngeal epithelia. Lancet 1:240–243
Zutter MM, Martin OJ, Sale GE, Shulman HM, Fisher L, Thomas ED, Durman DM (1988) Epstein-Barr virus lymphoprolifera-tion after bone marrow transplantation. Blood 72:520–529
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Ohshima, K., Kikuchi, M., Eguchi, F. et al. Analysis of Epstein-Barr viral genomes in lymphoid malignancy using southern blotting, polymerase chain reaction and in situ hybridization. Virchows Archiv B Cell Pathol 59, 383–390 (1990). https://doi.org/10.1007/BF02899428
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DOI: https://doi.org/10.1007/BF02899428